Title: | Repellent properties of Rotheca glabrum plant extracts against adults of Rhipicephalus appendiculatus |
Author(s): | Mawela KG; Luseba D; Magano S; Eloff JN; |
Address: | "Phytomedicine Programme, Department of Paraclinical Sciences, University of Pretoria, Private Bag X04, Onderstepoort, 0110, Republic of South Africa. Agricultural Research Council, Onderstepoort Veterinary Research, Division of Toxicology, Private Bag X05, Onderstepoort, 0110, South Africa. Department of Animal Sciences, Faculty of Science, Tshwane University of Technology, Private Bag X680, Pretoria, 0001, South Africa. Department of Life and Consumer Science, University of South Africa, Private Bag X6, Florida, 1710, Republic of South Africa. Department Agriculture and Animal Health, University of South Africa, Private Bag X6, Florida, 1710, Republic of South Africa. kobus.eloff@up.ac.za" |
DOI: | 10.1186/s12917-019-1853-5 |
ISSN/ISBN: | 1746-6148 (Electronic) 1746-6148 (Linking) |
Abstract: | "BACKGROUND: Rotheca glabrum (formerly known as Clerodendrum glabrum [Verbenaceae]) is used by local communities in the Limpopo Province of South Africa to control ticks on livestock and was selected from the database of the ARC-Onderstepoort Veterinary Institute. Its leaves were extracted using organic solvents ranging from polar to non-polar solvents (methanol, acetone and dichloromethane (DCM)). In addition, the traditional soap-water (infusion) and water-based (decoction) methods were used. The tick repelling activity was determined against the adult stage of the livestock tick Rhipicephalus appendiculatus. RESULTS: In the tick-climbing repellency bioassay a 30% acetone extract had a significant (p = 0.05) repellent effect against adults of R. appendiculatus. The extract was still active at a lower concentration of 10%. The hexane fraction from the R. glabrum acetone extract had a higher tick repellency activity than the positive controls Amitix and Bayticol at the same concentrations. Unfortunately, the activity decreased after 2.5 h, probably due to volatility of the biologically active compound(s) within the extract. CONCLUSION: Attempts were made to isolate the repellent compound from the acetone extract of R. glabrum. The process produced very good results up to a late stage in the bioassay-guided fractionation process. At that point, the repellent activity was lost. When two fractions were combined, the repellent activity was regained. These results provide strong evidence for the existence of a synergisticactivity of different compounds. It may be better to concentrate on extracts that would kill ticks rather than on extracts that would repel ticks" |
Keywords: | "Animals Behavior, Animal/drug effects Insect Repellents/chemistry/*pharmacology Plant Extracts/chemistry/*pharmacology Plant Leaves/*chemistry Rhipicephalus/*drug effects Verbenaceae/*chemistry Bioassay-guided fractionation Decoction Infusion Repellent Ro;" |
Notes: | "MedlineMawela, Kedibone Gloria Luseba, Dibungi Magano, Solomon Eloff, Jacobus Nicolaas eng Incentive Funding/National Research Foundation (ZA)/ England 2019/04/29 BMC Vet Res. 2019 Apr 27; 15(1):122. doi: 10.1186/s12917-019-1853-5" |