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J Cell Biol

Title:		Pheromone-induced polarization is dependent on the Fus3p MAPK acting through the formin Bni1p
Author(s):		Matheos D; Metodiev M; Muller E; Stone D; Rose MD;
Address:		"Department of Molecular Biology, Princeton University, Princeton, NJ 08544-1014, USA"
Journal Title:		J Cell Biol
Year:		2004
Volume:		20040405
Issue:		1
Page Number:		99 - 109
DOI:		10.1083/jcb.200309089
ISSN/ISBN:		0021-9525 (Print) 1540-8140 (Electronic) 0021-9525 (Linking)
Abstract:		"During mating, budding yeast cells reorient growth toward the highest concentration of pheromone. Bni1p, a formin homologue, is required for this polarized growth by facilitating cortical actin cable assembly. Fus3p, a pheromone-activated MAP kinase, is required for pheromone signaling and cell fusion. We show that Fus3p phosphorylates Bni1p in vitro, and phosphorylation of Bni1p in vivo during the pheromone response is dependent on Fus3p. fus3 mutants exhibited multiple phenotypes similar to bni1 mutants, including defects in actin and cell polarization, as well as Kar9p and cytoplasmic microtubule localization. Disruption of the interaction between Fus3p and the receptor-associated Galpha subunit caused similar mutant phenotypes. After pheromone treatment, Bni1p-GFP and Spa2p failed to localize to the cortex of fus3 mutants, and cell wall growth became completely unpolarized. Bni1p overexpression suppressed the actin assembly, cell polarization, and cell fusion defects. These data suggest a model wherein activated Fus3p is recruited back to the cortex, where it activates Bni1p to promote polarization and cell fusion"
Keywords:		"Actin Cytoskeleton/metabolism Cell Movement/genetics/*physiology Cell Polarity/genetics/*physiology Cell Size/genetics Cytoskeletal Proteins GTP-Binding Proteins/genetics/metabolism Gene Expression Regulation, Fungal/genetics Microfilament Proteins/geneti;"
Notes:		"MedlineMatheos, Dina Metodiev, Metodi Muller, Eric Stone, David Rose, Mark D eng R01 GM037739/GM/NIGMS NIH HHS/ GM37739/GM/NIGMS NIH HHS/ Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. 2004/04/07 J Cell Biol. 2004 Apr; 165(1):99-109. doi: 10.1083/jcb.200309089. Epub 2004 Apr 5"