| Title: | Functional Characterization of Hedychium coronarium J. Koenig MYB132 Confers the Potential Role in Floral Aroma Synthesis |
| Author(s): | Abbas F; Ke Y; Zhou Y; Yu R; Imran M; Amanullah S; Rothenberg DO; Wang Q; Wang L; Fan Y; |
| Address: | "The Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China. College of Economics and Management, Kunming University, Kunming 650214, China. College of Life Sciences, South China Agricultural University, Guangzhou 510642, China. Department of Crop Science and Technology, College of Agriculture, South China Agricultural University, Guangzhou 510642, China. College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China. College of Horticulture, South China Agricultural University, Guangzhou 510642, China. Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou 510642, China" |
| ISSN/ISBN: | 2223-7747 (Print) 2223-7747 (Electronic) 2223-7747 (Linking) |
| Abstract: | "The R2R3-MYB transcription factors (TFs) play several key roles in numerous plant biological processes. Hedychium coronarium is an important ornamental plant well-known for its elegant flower shape and abundant aroma type. The floral aroma of H. coronarium is due to the presence of a large amount of terpenes and benzenoids. However, less is known about the role of R2R3-MYB TFs in the regulatory mechanism of floral aroma production in this breed. Herein, we isolate and functionally characterize the R2R3-MYB TF HcMYB132, which is potentially involved in regulating floral aroma synthesis. Sequence alignment analysis revealed that it includes a nuclear localization signal NLS(s) and a 2R, 3R motif signature in the sequences. A subcellular localization assay revealed that HcMYB132 protein localizes to the nucleus. Real-time qPCR assays showed that HcMYB132 is specifically expressed in flowers and its expression pattern correlates with the emission of floral volatile compounds. In HcMYB132-silenced flowers, the levels of floral volatile compounds were significantly reduced, and the expression of key structural volatile synthesis genes was downregulated compared to control. Collectively, these results suggest that HcMYB132 might play a significant role in the regulation of terpenoid biosynthesis in H. coronarium" |
| Keywords: | Hedychium coronarium R2r3-myb floral scent structural genes terpenes; |
| Notes: | "PubMed-not-MEDLINEAbbas, Farhat Ke, Yanguo Zhou, Yiwei Yu, Rangcai Imran, Muhammad Amanullah, Sikandar Rothenberg, Dylan O'Neill Wang, Qin Wang, Lan Fan, Yanping eng 31870690/National Natural Science Foundation of China/ 31770738/National Natural Science Foundation of China/ 2018YFD1000407/National Key Research and Development Program of China/ 2020B020220007/Key-Areas Research and Development Program of Guangdong Province/ 201903010054/People's Livelihood Science and Technology Projects of Guangzhou/ Switzerland 2021/10/24 Plants (Basel). 2021 Sep 25; 10(10):2014. doi: 10.3390/plants10102014" |
