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Biochem Biophys Res Commun

Title:		Total in vitro maturation of the Saccharomyces cerevisiae a-factor lipopeptide mating pheromone
Author(s):		Marcus S; Caldwell GA; Xue CB; Naider F; Becker JM;
Address:		"Department of Microbiology, University of Tennessee, Knoxville 37996"
Journal Title:		Biochem Biophys Res Commun
Year:		1990
Volume:		172
Issue:		3
Page Number:		1310 - 1316
DOI:		10.1016/0006-291x(90)91592-g
ISSN/ISBN:		0006-291X (Print) 0006-291X (Linking)
Abstract:		"The a-factor mating pheromone, produced by Saccharomyces cerevisiae a haploid cells, is post-translationally modified in a manner analogous to that of the ras proto-oncogene product. A consensus C-terminal amino acid sequence, -CAAX (C is cysteine, A is aliphatic amino acid, and X is any amino acid), is the target of these modifications, which include isoprenylation (essential for Ras function), proteolysis of the -AAX sequence, and carboxy methyl esterification. Recently, the RAM/DPR1 gene product was shown to be a component of the activity responsible for isoprenylation of both Ras and a-factor. In this report, we present an in vitro assay which not only detects a-factor isoprenylation, but also proteolysis and carboxy methyl esterification, and directly demonstrates, biochemically, the order of these processing events. This a-factor maturation assay may prove useful for screening agents which block any of the steps involved in the post-translational modification of the a-factor and Ras -CAAX sequences. Such agents would be potential anti-Ras-related cancer therapeutic drugs"
Keywords:		"Amino Acid Sequence Antineoplastic Agents Base Sequence Consensus Sequence Mating Factor Methylation Molecular Sequence Data Mutation Peptides/*genetics Protein Processing, Post-Translational Proto-Oncogene Proteins/*genetics Saccharomyces cerevisiae/gene;"
Notes:		"MedlineMarcus, S Caldwell, G A Xue, C B Naider, F Becker, J M eng GM-22086/GM/NIGMS NIH HHS/ GM-22087/GM/NIGMS NIH HHS/ Research Support, U.S. Gov't, P.H.S. 1990/11/15 Biochem Biophys Res Commun. 1990 Nov 15; 172(3):1310-6. doi: 10.1016/0006-291x(90)91592-g"