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J Agric Food Chem

Title:		"Volatile release from whey protein isolate-pectin multilayer stabilized emulsions: effect of pH, salt, and artificial salivas"
Author(s):		Mao L; Roos YH; O'Callaghan DJ; Miao S;
Address:		"Teagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, Ireland"
Journal Title:		J Agric Food Chem
Year:		2013
Volume:		20130619
Issue:		26
Page Number:		6231 - 6239
DOI:		10.1021/jf4011615
ISSN/ISBN:		1520-5118 (Electronic) 0021-8561 (Linking)
Abstract:		"Whey protein isolate (WPI) and pectin can form a multilayer at the oil-water interface when they are oppositely charged. In this study, effects of pH, salt, and artificial salivas on emulsion stability and volatile release from multilayer emulsions were investigated. Results showed that emulsions (0.5 wt % WPI, 10 wt % oil) with pectin content /=0.2 wt %) had good stability. Due to an electrostatic screening effect, multilayer emulsions collapsed when subjected to >/=150 mM NaCl solutions at pH 5. When diluted with artificial salivas containing salts, mucin, and/or alpha-amylase, multilayer emulsions showed rapid droplet aggregation. GC headspace analysis found that volatiles had significantly lower initial headspace concentration (C(initial)) in multilayer emulsions, and the C(initial) correlated negatively with pectin content in emulsions. Emulsions at pH 7 had more volatiles released to the headspace than emulsions at pH 5. However, changes in pectin content and pH did not show a significant effect on release rate of most volatile compounds. In salt-treated multilayer emulsions, C(initial) and release rates of volatiles increased with NaCl content. Addition of salivas triggered higher release of hydrophobic volatiles and lower release of hydrophilic volatiles, which was mostly due to dilution effect and saliva-induced emulsion instability"
Keywords:		"Emulsions Humans Hydrogen-Ion Concentration Kinetics Milk Proteins/*chemistry/metabolism *Models, Biological Osmolar Concentration Pectins/*chemistry/metabolism Protein Stability Saliva/chemistry/*metabolism Sodium Chloride, Dietary/*analysis/metabolism T;"
Notes:		"MedlineMao, Like Roos, Yrjo H O'Callaghan, Donal J Miao, Song eng Comparative Study 2013/05/31 J Agric Food Chem. 2013 Jul 3; 61(26):6231-9. doi: 10.1021/jf4011615. Epub 2013 Jun 19"