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FEBS Lett

Title:		Oral secretions from herbivorous lepidopteran larvae exhibit ion channel-forming activities
Author(s):		Maischak H; Grigoriev PA; Vogel H; Boland W; Mithofer A;
Address:		"Max-Planck-Institut fur Chemische Okologie, Hans-Knoll-Str. 8, D-07745 Jena, Germany"
Journal Title:		FEBS Lett
Year:		2007
Volume:		20070205
Issue:		5
Page Number:		898 - 904
DOI:		10.1016/j.febslet.2007.01.067
ISSN/ISBN:		0014-5793 (Print) 0014-5793 (Linking)
Abstract:		"Feeding insects introduce oral secretions (OS) into the wounded tissue of the attacked plant. Various OS-derived molecules must be involved in subsequent processes including the induction of plant defence reactions. Using the planar lipid bilayer membrane technique, isolated OS were analyzed with respect to their membrane activities. Transmembrane ion fluxes were generated by OS of eight different lepidopteran larvae, all of which form comparable ion channels in artificial membranes. Currents were characterized by long lasting open times and conductivities from 250pS up to 1100pS. Channels formed by Spodoptera exigua secretions showed a preference for cations over anions. OS also induced a transient increase of the cytosolic calcium concentration in soybean cells, determined by the aequorin technique. Known compounds of the OS, fatty-acid-glutamine conjugates, also interfered with the membrane but were unable to form stable channels. Since ion fluxes and depolarization are early responses upon insect feeding, OS-derived components may be involved in the elicitation process by direct interaction with the plant membranes"
Keywords:		"Aequorin/genetics/metabolism Animals Calcium/metabolism In Vitro Techniques Ion Channels/*drug effects Lepidoptera/*physiology Lipid Bilayers Manduca/physiology Plants, Genetically Modified Soybeans/drug effects/metabolism/parasitology Spodoptera/physiolo;"
Notes:		"MedlineMaischak, Heiko Grigoriev, Pavel A Vogel, Heiko Boland, Wilhelm Mithofer, Axel eng Research Support, Non-U.S. Gov't England 2007/02/13 FEBS Lett. 2007 Mar 6; 581(5):898-904. doi: 10.1016/j.febslet.2007.01.067. Epub 2007 Feb 5"