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Front Microbiol

Title:		The transcription factor Ste12-like increases the mycelial abiotic stress tolerance and regulates the fruiting body development of Flammulina filiformis
Author(s):		Lyu X; Wang Q; Liu A; Liu F; Meng L; Wang P; Zhang Y; Wang L; Li Z; Wang W;
Address:		"Shandong Provincial Key Laboratory of Agricultural Microbiology, College of Plant Protection, Shandong Agricultural University, Tai'an, China. Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China"
Journal Title:		Front Microbiol
Year:		2023
Volume:		20230504
Issue:
Page Number:		1139679 -
DOI:		10.3389/fmicb.2023.1139679
ISSN/ISBN:		1664-302X (Print) 1664-302X (Electronic) 1664-302X (Linking)
Abstract:		"INTRODUCTION: Flammulina filiformis is one of the most commercially important edible fungi worldwide, with its nutritional value and medicinal properties. It becomes a good model species to study the tolerance of abiotic stress during mycelia growth in edible mushroom cultivation. Transcription factor Ste12 has been reported to be involved in the regulation of stress tolerance and sexual reproduction in fungi. METHODS: In this study, identification and phylogenetic analysis of ste12-like was performed by bioinformatics methods. Four ste12-like overexpression transformants of F. filiformis were constructed by Agrobacterium tumefaciens-mediated transformation. RESULTS AND DISCUSSION: Phylogenetic analysis showed that Ste12-like contained conserved amino acid sequences. All the overexpression transformants were more tolerant to salt stress, cold stress and oxidative stress than wild-type strains. In the fruiting experiment, the number of fruiting bodies of overexpression transformants increased compared with wild-type strains, but the growth rate of stipes slowed down. It suggested that gene ste12-like was involved in the regulation of abiotic stress tolerance and fruiting body development in F. filiformis"
Keywords:		Mapk abiotic stress pheromone signaling pathway transcription factor winter mushroom;
Notes:		"PubMed-not-MEDLINELyu, Xiaomeng Wang, Qingji Liu, Ao Liu, Fang Meng, Li Wang, Panmeng Zhang, Yan Wang, Li Li, Zhuang Wang, Wei eng Switzerland 2023/05/22 Front Microbiol. 2023 May 4; 14:1139679. doi: 10.3389/fmicb.2023.1139679. eCollection 2023"