| Title: | Olfactory biosensor for insect semiochemicals analysis by impedance sensing of odorant-binding proteins on interdigitated electrodes |
| Author(s): | Lu Y; Yao Y; Zhang Q; Zhang D; Zhuang S; Li H; Liu Q; |
| Address: | "Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, P.R. China. College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, P.R. China. College of Life Sciences, China Jiliang University, Hangzhou 310018, P.R. China. Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, P.R. China. Electronic address: qjliu@zju.edu.cn" |
| DOI: | 10.1016/j.bios.2014.09.098 |
| ISSN/ISBN: | 1873-4235 (Electronic) 0956-5663 (Linking) |
| Abstract: | "Insects can sensitively and selectively detect thousands of semiochemicals at very low concentrations by their remarkable olfactory systems. As one of the most important olfactory proteins, odorant-binding proteins (OBPs) from insects are the most promising candidates for fabricating biosensors to detect biochemical molecules in the chemical ecology as well as for other biotechnological applications. In this study, we designed an olfactory biosensor by immobilizing OBPs from oriental fruit fly on interdigitated electrodes to detect semiochemicals. After successfully separated and purified, OBPs were immobilized by the special designed polyethylene glycol (PEG), SH-PEG-COOH, to produce a robust sensing membrane. Based on electrochemical sensing, interactions between OBPs and different semiochemicals emitted from host plants of the insect, such as the isoamyl acetate, beta-ionone, and benzaldehyde, could be sensitively detected. With related amino acid residues in the hydrophobic cavities distinguished, the interaction forces between semiochemicals and OBPs were analyzed by molecular docking. Integrated biological olfaction proteins of insects, OBPs based biosensors could not only advance the progress in the understanding of chemical communication systems of insects, but also show promising potentials for biosensing applications in many fields" |
| Keywords: | "Amino Acid Sequence Animals *Biosensing Techniques Electric Impedance Electrodes Immobilized Proteins/chemistry Insect Proteins/*chemistry Molecular Docking Simulation Pheromones/chemistry/*isolation & purification Receptors, Odorant/*chemistry Smell/phys;" |
| Notes: | "MedlineLu, Yanli Yao, Yao Zhang, Qian Zhang, Diming Zhuang, Shulin Li, Hongliang Liu, Qingjun eng Research Support, Non-U.S. Gov't England 2014/12/03 Biosens Bioelectron. 2015 May 15; 67:662-9. doi: 10.1016/j.bios.2014.09.098. Epub 2014 Oct 7" |
