| Title: | Aroma volatiles obtained at harvest by HS-SPME/GC-MS and INDEX/MS-E-nose fingerprint discriminate climacteric behaviour in melon fruit |
| Author(s): | Chaparro-Torres LA; Bueso MC; Fernandez-Trujillo JP; |
| Address: | "Department of Agricultural & Food Engineering, Regional Campus of International Excellence 'Campus Mare Nostrum' (CMN), Technical University of Cartagena (UPCT), Paseo Alfonso XIII, 48, ETSIA, E-30203, Cartagena, Murcia, Spain. Institute of Plant Biotechnology, CMN, UPCT, Campus Muralla del Mar, Plaza del Hospital s/n, E-30202, Cartagena, Murcia, Spain. Department of Applied Mathematics and Statistics, CMN, UPCT, Doctor Fleming s/n, ETSII, E-30202, Cartagena, Murcia, Spain" |
| ISSN/ISBN: | 1097-0010 (Electronic) 0022-5142 (Linking) |
| Abstract: | "BACKGROUND: Melon aroma volatiles were extracted at harvest from juice of a climacteric near-isogenic line (NIL) SC3-5-1 with two quantitative trait loci (QTLs) introgressed which produced climacteric behaviour and its non-climacteric parental (PS) using two methodologies of analysis: static headspace solid phase micro-extraction (HS-SPME) by gas chromatography-mass spectrometry (GC-MS) and inside needle dynamic extraction (INDEX) by MS-based electronic nose (MS-E-nose). RESULTS: Of the 137 volatiles compounds identified, most were found at significantly higher concentrations in SC3-5-1 than in PS in both seasons. These volatiles were mostly esters, alcohols, sulfur-derived esters and even some aldehydes and others. The number of variables with high correlation values was reduced by using correlation network analysis. Partial least squares-discriminant analysis (PLS-DA) achieved the correct classification of PS and SC3-5-1. The ions m/z 74, 91, 104, 105, 106 and 108, mainly volatile derivatives precursor phenylalanine, were the most discriminant in SC3-5-1 and PS. As many as 104 QTLs were mapped in season 1 and at least 78 QTLs in each season with an effect above the PS mean. CONCLUSION: GC-MS gave better discrimination than E-nose. Most of the QTLs that mapped in both seasons enhanced aroma volatiles associated with climacteric behaviour" |
| Keywords: | Cucurbitaceae/*chemistry/genetics Food Analysis Fruit/*chemistry Gas Chromatography-Mass Spectrometry/*methods Odorants/*analysis Volatile Organic Compounds/*chemistry Cucumis melo L aroma extraction aroma fingerprint multivariate statistical techniques n; |
| Notes: | "MedlineChaparro-Torres, Libia A Bueso, Maria C Fernandez-Trujillo, Juan P eng Research Support, Non-U.S. Gov't England 2015/07/28 J Sci Food Agric. 2016 May; 96(7):2352-65. doi: 10.1002/jsfa.7350. Epub 2015 Sep 3" |
