| Title: | A New Method for Determination of Thymol and Carvacrol in Thymi herba by Ultraperformance Convergence Chromatography (UPC(2)) |
| Author(s): | Chang X; Sun P; Ma Y; Han D; Zhao Y; Bai Y; Zhang D; Yang L; |
| Address: | "Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. Artemisinin Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China" |
| DOI: | 10.3390/molecules25030502 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "Ultraperformance convergence chromatography is an environmentally friendly analytical technique for dramatically reducing the use of organic solvents compared to conventional chromatographic methods. In this study, a rapid and sensitive ultraperformance convergence chromatography method was firstly established for quantification of thymol and carvacrol, two positional isomers of a major bioactive in the volatile oil of Thymi herba, the dried leaves and flowers of Thymus mongolicus or Thymus przewalskii, known in China as 'Dijiao.' Using a Trefoil(TM) CEL1 column, thymol and carvacrol were separated in less than 2.5 min and resolution was enhanced. The method was validated with respect to precision, accuracy, and linearity according to the National Medical Products Administration guidelines. The optimized method exhibited good linear correlation (r = 0.9998-0.9999), excellent precision (relative standard deviations (RSDs) < 1.50%), and acceptable recoveries (87.29-102.89%). The limits of detection for thymol and carvacrol were 1.31 and 1.57 ng/L, respectively, while their corresponding limits of quantification were 2.63 and 3.14 ng/L. Finally, the quantities of the two compounds present in 16 T. mongolicus and four T. przewalskii samples were successfully evaluated by employing the developed method. It is hoped that the results of this study will serve as a guideline for the quality control of Thymi herba" |
| Keywords: | "China Chromatography/instrumentation/*methods Cymenes/*analysis/chemistry/isolation & purification Flowers/chemistry Isomerism Oils, Volatile/chemistry Plant Leaves/chemistry Thymol/*analysis/chemistry/isolation & purification Thymus Plant/*chemistry Thym;" |
| Notes: | "MedlineChang, Xiaoqiang Sun, Peng Ma, Yue Han, Dongchen Zhao, Yifan Bai, Yue Zhang, Dong Yang, Lan eng 2060302/The ability establishment of sustainable use for valuable Chinese medicine resources/ Switzerland 2020/01/26 Molecules. 2020 Jan 23; 25(3):502. doi: 10.3390/molecules25030502" |
