| Title: | Silencers and domains of generalized repression |
| Address: | "Department of Molecular and Cell Biology, University of California at Berkeley 94720" |
| ISSN/ISBN: | 0036-8075 (Print) 0036-8075 (Linking) |
| Abstract: | "Gene expression can be affected by the chromosomal position of the gene. An example of this position effect is silencing of the HML and HMR mating-type loci of Saccharomyces cerevisiae. An in vitro assay revealed that silencing induced a transcription-independent general occlusion of the DNA at HMR from sequence-specific interactions of proteins with DNA. The minimum boundaries of the silenced chromatin structure were determined, as were the contributions of the E and I silencers to the size of the silenced domain. Examination of endonuclease-sensitive sites provided evidence that neither the integrity of the chromosomal duplex nor covalent linkage of the silencers to HMR was important for maintenance of the silenced structure in vitro" |
| Keywords: | "Cell Nucleus/metabolism Chromatin/*metabolism DNA Restriction Enzymes/metabolism DNA, Fungal/genetics/metabolism Deoxyribonucleases, Type II Site-Specific/metabolism *Gene Expression Regulation, Fungal Genes, Fungal Genes, Mating Type, Fungal Genes, Regul;" |
| Notes: | "MedlineLoo, S Rine, J eng GM31105/GM/NIGMS NIH HHS/ P30ESO1896-12/ES/NIEHS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1994/06/17 Science. 1994 Jun 17; 264(5166):1768-71. doi: 10.1126/science.8209257" |
