| Title: | Palmitoyl-protein thioesterase 1 (PPT1): an obesity-induced rat testicular marker of reduced fertility |
| Author(s): | Liu Y; Zhao W; Gu G; Lu L; Feng J; Guo Q; Ding Z; |
| Address: | "Department of Human Anatomy, Histology and Embryology, Shanghai Key Laboratory for Reproductive Medicine, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200025, China" |
| ISSN/ISBN: | 1098-2795 (Electronic) 1040-452X (Linking) |
| Abstract: | "Male obesity may lead to declines in testosterone levels, reproductive hormonal profile, and semen quantity. To assess the effects of obesity on spermatogenesis, Sprague-Dawley rats fed a high-fat diet served as a model of induced obesity. The litter sizes for females mated to obese males were significantly lower as compared to females mated with normal-diet-fed controls. Their serum high-density lipoprotein, low-density lipoprotein, cholesterol, and estradiol levels increased in obese males, but testosterone and follicle-stimulating hormone levels decreased. Testicular morphology disruptions included Sertoli-cell atrophy, disrupted tight junctions, and mitochondrial degeneration in spermatogenic cells. To further investigate the molecular mechanisms leading to high-fat-diet-induced changes, we employed testicular proteomic analysis on rats fed both types of diet. Three spots were up-regulated in rats fed a high-fat diet whereas two others were downregulated. One of the upregulated spots was palmitoyl-protein thioesterase 1 (PPT1), a lipoprotein metabolizing related enzyme localized to Sertoli cells. In a Sertoli-cell line cultured in a high-fat supplemented medium, PPT1 abundance was accompanied by increases in the endocytic vesicle-associated protein, clathrin, and decreases in the tight junctional proteins, ZO-1 and occludin. In conclusion, declines in rat male fertility induced by a high-fat diet are associated with an altered testicular protein expression pattern as well as disruption of testicular Sertoli-cell and spermatogenic-cell morphology. PPT1 expression may provide a testicular marker of reduced fertility in obese males, as increases in its expression may be detrimental to Sertoli-cell function during spermatogenesis" |
| Keywords: | "Analysis of Variance Animals Biomarkers/*metabolism Body Weight DNA Primers/genetics Electrophoresis, Gel, Two-Dimensional Fluorescent Antibody Technique Follicle Stimulating Hormone/blood Gene Expression Regulation/*physiology Infertility, Male/etiology/;" |
| Notes: | "MedlineLiu, Yue Zhao, Wenzhen Gu, Guobao Lu, Liming Feng, Jingsheng Guo, Qiangsu Ding, Zhide eng Research Support, Non-U.S. Gov't 2013/12/05 Mol Reprod Dev. 2014 Jan; 81(1):55-65. doi: 10.1002/mrd.22281. Epub 2013 Dec 3" |
