| Title: | Ssc-novel-miR-106-5p reduces lipopolysaccharide-induced inflammatory response in porcine endometrial epithelial cells by inhibiting the expression of the target gene mitogen-activated protein kinase kinase kinase 14 (MAP3K14) |
| Author(s): | Lian Y; Hu Y; Gan L; Huo YN; Luo HY; Wang XZ; |
| Address: | "Chongqing Key Laboratory of Forage and Herbivore, College of Animal Science and Technology, Southwest University, Beibei, Chongqing 400716, P. R. China. College of Resource and Environment, Southwest University, Beibei, Chongqing 400716, P. R. China. Chongqing Key Laboratory of Forage and Herbivore, College of Animal Science and Technology, Southwest University, Beibei, Chongqing 400716, P. R. China; and Corresponding author. Email: xianzhong_wang@aliyun.com" |
| ISSN/ISBN: | 1031-3613 (Print) 1031-3613 (Linking) |
| Abstract: | "As an important gram-negative bacterial outer membrane component, lipopolysaccharide (LPS) plays an important role in bacterial-induced endometritis in sows. However, how LPS induces endometritis is unclear. We stimulated sow endometrial epithelial cells (EECs) with LPS and detected cell viability and tumour necrosis factor-alpha (TNF-alpha) and interleukin-1 (IL-1) secretion. LPS affected EEC viability and TNF-alpha and IL-1 secretion in a dose-dependent manner. LPS induced differential expression in 10 of 393 miRNAs in the EECs (downregulated, nine; upregulated, one). MicroRNA (miRNA) high-throughput sequencing of the LPS-induced EECs plus bioinformatics analysis and the dual-luciferase reporter system revealed a novel miRNA target gene: mitogen-activated protein kinase kinase kinase 14 (MAP3K14). Ssc-novel-miR-106-5p mimic, inhibitor and the nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IkappaBalpha) phosphorylation inhibitor Bay11-7085 were used to detect EEC nuclear factor-kappaB phosphorylation levels (p-NF-kappaB) and TNF-alpha and IL-1 secretion. MiR-106-5p mimic downregulated MAP3K14 mRNA and protein expression levels, inhibited p-NF-kappaB levels and decreased IL-1 and TNF-alpha secretion, whereas miR-106-5p inhibitor had the opposite effect. Bay11-7085 inhibited p-NF-kappaB expression and TNF-alpha and IL-1 secretion. These results suggest that LPS downregulates ssc-novel-miR-106-5p expression in sow EECs to increase MAP3K14 expression, which increases p-NF-kappaB to promote IL-1 and TNF-alpha secretion" |
| Keywords: | "Animals Cells, Cultured Down-Regulation/drug effects/genetics Endometritis/chemically induced/genetics/metabolism/veterinary Endometrium/*drug effects/immunology/metabolism Epithelial Cells/*drug effects/immunology/metabolism Female Gene Expression Regula;" |
| Notes: | "MedlineLian, Yu Hu, Yu Gan, Lu Huo, Yuan-Nan Luo, Hong-Yan Wang, Xian-Zhong eng Australia 2019/06/28 Reprod Fertil Dev. 2019 Sep; 31(10):1616-1627. doi: 10.1071/RD19097" |
