| Title: | Over-expression of the red plant gene R1 enhances anthocyanin production and resistance to bollworm and spider mite in cotton |
| Author(s): | Li X; Ouyang X; Zhang Z; He L; Wang Y; Li Y; Zhao J; Chen Z; Wang C; Ding L; Pei Y; Xiao Y; |
| Address: | "Biotechnology Research Center, Southwest University, Chongqing, China. College of Agronomy and Biological Science and Technology, Southwest University, Chongqing, China. College of Plant Protection, Southwest University, Chongqing, China. Biotechnology Research Center, Southwest University, Chongqing, China. xiaoyuehua@swu.edu.cn" |
| DOI: | 10.1007/s00438-018-1525-3 |
| ISSN/ISBN: | 1617-4623 (Electronic) 1617-4623 (Linking) |
| Abstract: | "Anthocyanins are a class of pigments ubiquitously distributed in plants and play roles in adoption to several stresses. The red plant gene (R1) promotes light-induced anthocyanin accumulation and red/purple pigmentation in cotton. Using 11 markers developed via genome resequencing, the R1 gene was located in an interval of approximately 136 kb containing three annotated genes. Among them, a PAP1 homolog, GhPAP1D (Gohir.D07G082100) displayed differential transcript level in the red- and green-plant leaves. GhPAP1D encoded a R2R3-MYB transcription factor and its over-expression resulted in increased anthocyanin accumulation in transgenic tobaccos and cottons. Dual luciferase assay indicated that GhPAP1D activated the promoters of several cotton anthocyanin structural genes in tobacco leaves. Importantly, we found that the GhPAP1D-overexpressing cotton leaves had increased resistance to both bollworm and spite mite. Our data demonstrated that GhPAP1D was the controlling gene of the red plant phenotype in cotton, and as the major anthocyanin regulator, this gene was potential to create transgenic cottons with resistance to a broad spectrum of herbivores" |
| Keywords: | "Animals Anthocyanins/*genetics/metabolism Disease Resistance/*genetics Gene Expression Regulation, Plant Genome, Plant Gossypium/*genetics/growth & development Helminths/genetics Pest Control, Biological Pigmentation/genetics Plant Leaves/*genetics/growth;" |
| Notes: | "MedlineLi, Xin Ouyang, Xufen Zhang, Zhengsheng He, Lin Wang, Yi Li, Yaohua Zhao, Jia Chen, Zhong Wang, Chuannan Ding, Lingli Pei, Yan Xiao, Yuehua eng 2016ZX08005005-001/Genetically Modified Organisms Breeding Major Project of China/ 31571582/National Natural Science Foundation of China/ 31871539/National Natural Science Foundation of China/ cstc2015jcyjA80001/Chongqing Project of Basic and Frontier Research/ Germany 2019/01/04 Mol Genet Genomics. 2019 Apr; 294(2):469-478. doi: 10.1007/s00438-018-1525-3. Epub 2019 Jan 2" |
