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J Vis Exp

Title:		Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry
Author(s):		Li X; Huang DD; Du R; Zhang ZJ; Chan CK; Huang ZX; Zhou Z;
Address:		"Institute of Mass Spectrometer and Atmospheric Environment, Jinan University; Guangdong Provincial Engineering Research Center for On-line Source Apportionment System of Air Pollution; tamylee@jnu.edu.cn. School of Energy and Environment, City University of Hong Kong. Institute of Mass Spectrometer and Atmospheric Environment, Jinan University; Guangdong Provincial Engineering Research Center for On-line Source Apportionment System of Air Pollution. Institute of Mass Spectrometer and Atmospheric Environment, Jinan University"
Journal Title:		J Vis Exp
Year:		2018
Volume:		20180309
Issue:		133
Page Number:		-
DOI:		10.3791/56465
ISSN/ISBN:		1940-087X (Electronic) 1940-087X (Linking)
Abstract:		"Exhaled volatile organic compounds (VOCs) have aroused considerable interest, since they can serve as biomarkers for disease diagnosis and environmental exposure in a non-invasive manner. In this work, we present a protocol to characterize the exhaled VOCs in real time by using secondary nanoelectrospray ionization coupled to high resolution mass spectrometry (Sec-nanoESI-HRMS). The homemade Sec-nanoESI source was readily set up based on a commercial nanoESI source. Hundreds of peaks were observed in the background-subtracted mass spectra of exhaled breath, and the mass accuracy values are -4.0-13.5 ppm and -20.3-1.3 ppm in the positive and negative ion detection modes, respectively. The peaks were assigned with accurate elemental composition according to the accurate mass and isotopic pattern. Less than 30 s is used for one exhalation measurement, and it takes approximately 7 min for six replicated measurements"
Keywords:		"Breath Tests/*methods Environmental Exposure/analysis Humans Mass Spectrometry/*methods Spectrometry, Mass, Electrospray Ionization/*methods Volatile Organic Compounds/pharmacology/*therapeutic use;"
Notes:		"MedlineLi, Xue Huang, Dan D Du, Rui Zhang, Zhi J Chan, Chak K Huang, Zheng X Zhou, Zhen eng Research Support, Non-U.S. Gov't Video-Audio Media 2018/03/27 J Vis Exp. 2018 Mar 9; (133):56465. doi: 10.3791/56465"