| Title: | Tissue specificity of (E)-beta-farnesene and germacrene D accumulation in pyrethrum flowers |
| Author(s): | Li J; Hu H; Chen Y; Xie J; Li J; Zeng T; Wang M; Luo J; Zheng R; Jongsma MA; Wang C; |
| Address: | "Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China. Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China. Business Unit Bioscience, Wageningen University and Research, Droevendaalsesteeg 1, 6708, PB Wageningen, the Netherlands. Electronic address: maarten.jongsma@wur.nl. Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China. Electronic address: wangcy@mail.hzau.edu.cn" |
| DOI: | 10.1016/j.phytochem.2021.112768 |
| ISSN/ISBN: | 1873-3700 (Electronic) 0031-9422 (Linking) |
| Abstract: | "Plant defensive mimicry based on the aphid alarm pheromone (E)-beta-farnesene (EbetaF) was previously shown to operate in Tanacetum cinerariifolium (Asteraceae) flowers. Germacrene D (GD), is another dominant volatile of T. cinerariifolium flowers and may modulate both defense and pollination. Here, we find that the increase in GD/EbetaF ratio at later developmental stages is correlated with the tissue distribution in the flower head: the total content of EbetaF and GD is similar, but GD accumulates comparatively more in the upper disk florets. Naphthol and N, N-dimethyl-p-phenylenediamine dihydrochloride (NADI)-stained purple ducts containing EbetaF and GD, were observed in the five petal lips of the corolla and two-lobed stigma of disk florets. By contrast in the peduncle, EbetaF accounts for nearly 80% of total terpenes, compared to 5% for GD. EbetaF is accumulated inside inner cortex cells and parenchyma cells of the pith in young peduncle. This is followed by the formation of terpene-filled axial secretory cavities parallel to the vascular bundles. In conclusion, the observed developmental and diurnal emissions of different EbetaF/GD ratios appear to be regulated by their tissue distribution" |
| Keywords: | "Animals *Aphids *Chrysanthemum cinerariifolium Flowers Organ Specificity *Sesquiterpenes Sesquiterpenes, Germacrane (E)-beta-farnesene Asteraceae Germacrene D NADI staining Pyrethrum Secretory duct Tanacetum cinerariifolium Terpenes;" |
| Notes: | "MedlineLi, Jinjin Hu, Hao Chen, Yu Xie, Jing Li, Jiawen Zeng, Tuo Wang, Manqun Luo, Jing Zheng, Riru Jongsma, Maarten A Wang, Caiyun eng England 2021/05/02 Phytochemistry. 2021 Jul; 187:112768. doi: 10.1016/j.phytochem.2021.112768. Epub 2021 Apr 28" |
