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Bioprocess Biosyst Eng

Title:		Ranking the significance of fermentation conditions on the volatile organic compounds of Tuber melanosporum fermentation system by combination of head-space solid phase microextraction and chromatographic fingerprint similarity analysis
Author(s):		Li DC; Liu RS; Li HM; Yuan ZP; Chen T; Tang YJ;
Address:		"Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei University of Technology, Wuhan, 430068, China"
Journal Title:		Bioprocess Biosyst Eng
Year:		2014
Volume:		20130813
Issue:		3
Page Number:		543 - 552
DOI:		10.1007/s00449-013-1021-4
ISSN/ISBN:		1615-7605 (Electronic) 1615-7591 (Linking)
Abstract:		"Tuber melanosporum is highly appreciated in culinary contexts due to its unique and characteristic aroma. T. melanosporum fermentation has been established as a promising alternative for fruiting bodies to produce volatile organic compounds (VOCs). In this work, a technique using a combination of chromatographic fingerprint similarity analysis, head-space solid phase microextraction and gas chromatography was developed to rank the significance of fermentation conditions on the VOCs profile during T. melanosporum fermentation. Omission tests indicated that the absence of major carbon source (i.e., sucrose) in the fermentation media had the most significant effect on the profile of VOCs, followed by the absence of yeast extract or peptone. Consideration of the culture conditions revealed that VOCs produced was the most significantly affected by temperature. These results indicated that it is possible to adjust the aroma of truffles via fermentation process control"
Keywords:		"Ascomycota/*chemistry Chromatography, Gas/*methods *Fermentation Hydrogen-Ion Concentration Solid Phase Microextraction/*methods Temperature Volatile Organic Compounds/*metabolism;"
Notes:		"MedlineLi, Dao-Cheng Liu, Rui-Sang Li, Hong-Mei Yuan, Zhan-Peng Chen, Tao Tang, Ya-Jie eng Research Support, Non-U.S. Gov't Germany 2013/08/15 Bioprocess Biosyst Eng. 2014 Mar; 37(3):543-52. doi: 10.1007/s00449-013-1021-4. Epub 2013 Aug 13"