| Title: | Resistance of cultivated tomato to cell content-feeding herbivores is regulated by the octadecanoid-signaling pathway |
| Author(s): | Li C; Williams MM; Loh YT; Lee GI; Howe GA; |
| Address: | "Department of Energy-Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824, USA" |
| ISSN/ISBN: | 0032-0889 (Print) 1532-2548 (Electronic) 0032-0889 (Linking) |
| Abstract: | "The octadecanoid signaling pathway has been shown to play an important role in plant defense against various chewing insects and some pathogenic fungi. Here, we examined the interaction of a cell-content feeding arachnid herbivore, the two-spotted spider mite (Tetranychus urticae Koch), with cultivated tomato (Lycopersicon esculentum) and an isogenic mutant line (defenseless-1 [def-1]) that is deficient in the biosynthesis of the octadecanoid pathway-derived signal, jasmonic acid (JA). Spider mite feeding and fecundity on def-1 plants was significantly greater than on wild-type plants. Decreased resistance of def-1 plants was correlated with reduced JA accumulation and expression of defensive proteinase inhibitor (PI) genes, which were induced in mite-damaged wild-type leaves. Treatment of def-1 plants with methyl-JA restored resistance to spider mite feeding and reduced the fecundity of female mites. Plants expressing a 35S::prosystemin transgene that constitutively activates the octadecanoid pathway in a Def-1-dependent manner were highly resistant to attack by spider mites and western flower thrips (Frankliniella occidentalis), another cell-content feeder of economic importance. These findings indicate that activation of the octadecanoid signaling pathway promotes resistance of tomato to a broad spectrum of herbivores. The techniques of amplified fragment length polymorphism (AFLP) and bulk segregant analysis were used to map the Def-1 gene to a region on the long arm of chromosome 3 that is genetically separable from the map position of known JA biosynthetic genes. Tight linkage of Def-1 to a T-DNA insertion harboring the maize (Zea mays) Dissociation transposable element suggests a strategy for directed transposon tagging of the gene" |
| Keywords: | "Acetates/pharmacology Animals Arachnida/*growth & development Chromosome Mapping Cyclopentanes/metabolism/pharmacology Female Genotype Immunity, Innate/drug effects Insecta/growth & development Solanum lycopersicum/drug effects/genetics/*parasitology Muta;" |
| Notes: | "MedlineLi, Chuanyou Williams, Mark M Loh, Ying-Tsu Lee, Gyu In Howe, Gregg A eng R01 GM057795/GM/NIGMS NIH HHS/ GM57795/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. 2002/09/13 Plant Physiol. 2002 Sep; 130(1):494-503. doi: 10.1104/pp.005314" |
