| Title: | A transcriptional mediator protein that is required for activation of many RNA polymerase II promoters and is conserved from yeast to humans |
| Author(s): | Lee YC; Min S; Gim BS; Kim YJ; |
| Address: | "Center for Basic Research, Samsung Biomedical Research Institute, Kangnam-ku, Seoul, Korea" |
| ISSN/ISBN: | 0270-7306 (Print) 1098-5549 (Electronic) 0270-7306 (Linking) |
| Abstract: | "A temperature-sensitive mutation was obtained in Med6p, a component of the mediator complex from the yeast Saccharomyces cerevisiae. The mediator complex has been shown to enable transcriptional activation in vitro. This mutation in Med6p abolished activation of transcription from four of five inducible promoters tested in vivo. There was no effect, however, on uninduced transcription, transcription of constitutively expressed genes, or transcription by RNA polymerases I and III. Mediator-RNA polymerase II complex isolated from the mutant yeast strain was temperature sensitive for transcriptional activation in a reconstituted in vitro system due to a defect in initiation complex formation. A database search revealed the existence of MED6-related genes in humans and Caenorhabditis elegans, suggesting that the role of mediator in transcriptional activation is conserved throughout the evolution" |
| Keywords: | "Amino Acid Sequence Carrier Proteins/genetics Cell Extracts Cell Nucleus/metabolism Cloning, Molecular Coenzymes DNA-Directed RNA Polymerases/metabolism Fungal Proteins/*genetics/metabolism/physiology Gene Expression Regulation, Fungal/physiology Genes, F;" |
| Notes: | "MedlineLee, Y C Min, S Gim, B S Kim, Y J eng 1997/08/01 Mol Cell Biol. 1997 Aug; 17(8):4622-32. doi: 10.1128/MCB.17.8.4622" |
