| Title: | The volatile metabolome of grapevine roots: first insights into the metabolic response upon phylloxera attack |
| Author(s): | Lawo NC; Weingart GJ; Schuhmacher R; Forneck A; |
| Address: | "Division of Viticulture and Pomology, Department of Crop Sciences, University of Natural Resources and Life Sciences, Konrad-Lorenz Str. 24, 3430 Tulln, Austria. nora.lawo@gmx.de" |
| DOI: | 10.1016/j.plaphy.2011.06.008 |
| ISSN/ISBN: | 1873-2690 (Electronic) 0981-9428 (Print) 0981-9428 (Linking) |
| Abstract: | "Many plant species respond to herbivore attack by an increased formation of volatile organic compounds. In this preliminary study we analysed the volatile metabolome of grapevine roots [Teleki 5C (Vitis berlandieri Planch. x Vitis riparia Michx.)] with the aim to gain insight into the interaction between phylloxera (Daktulosphaira vitifoliae Fitch; Hemiptera: Phylloxeridae) and grapevine roots. In the first part of the study, headspace solid phase microextraction (HS-SPME) coupled to gas chromatography - mass spectrometry (GC-MS) was used to detect and identify volatile metabolites in uninfested and phylloxera-infested root tips of the grapevine rootstock Teleki 5C. Based on the comparison of deconvoluted mass spectra with spectra databases as well as experimentally derived retention indices with literature values, 38 metabolites were identified, which belong to the major classes of plant volatiles including C6-compounds, terpenes (including modified terpenes), aromatic compounds, alcohols and n-alkanes. Based on these identified metabolites, changes in root volatiles were investigated and resulted in metabolite profiles caused by phylloxera infestation. Our preliminary data indicate that defence related pathways such as the mevalonate and/or alternative isopentenyl pyrophosphate-, the lipoxygenase- (LOX) as well as the phenylpropanoid pathway are affected in root galls as a response to phylloxera attack" |
| Keywords: | Animals *Hemiptera *Metabolome Plant Diseases/*parasitology Plant Roots/*metabolism/*parasitology Vitis/*metabolism/*parasitology Volatile Organic Compounds/analysis/*metabolism; |
| Notes: | "MedlineLawo, Nora C Weingart, Georg J F Schuhmacher, Rainer Forneck, Astrid eng P 21203/FWF_/Austrian Science Fund FWF/Austria Research Support, Non-U.S. Gov't France 2011/07/19 Plant Physiol Biochem. 2011 Sep; 49(9):1059-63. doi: 10.1016/j.plaphy.2011.06.008. Epub 2011 Jul 1" |
