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Plant Cell

Title:		VIH2 Regulates the Synthesis of Inositol Pyrophosphate InsP8 and Jasmonate-Dependent Defenses in Arabidopsis
Author(s):		Laha D; Johnen P; Azevedo C; Dynowski M; Weiss M; Capolicchio S; Mao H; Iven T; Steenbergen M; Freyer M; Gaugler P; de Campos MK; Zheng N; Feussner I; Jessen HJ; Van Wees SC; Saiardi A; Schaaf G;
Address:		"Center for Plant Molecular Biology, University of Tubingen, 72076 Tubingen, Germany. Medical Research Council Laboratory for Molecular Cell Biology, University College London, London WC1E 6BT, United Kingdom. Zentrum fur Datenverarbeitung, University of Tubingen, 72074 Tubingen, Germany. Department of Biology, University of Tubingen, 72076 Tubingen, Germany. Department of Chemistry, University of Zurich, 8057 Zurich, Switzerland. Department of Pharmacology, Howard Hughes Medical Institute, University of Washington, Seattle, Washington 98195. Department of Plant Biochemistry, Georg-August-University Gottingen, 37077 Gottingen, Germany. Plant-Microbe Interactions, Department of Biology, Utrecht University, 3508 TB Utrecht, The Netherlands. Center for Plant Molecular Biology, University of Tubingen, 72076 Tubingen, Germany gabriel.schaaf@zmbp.uni-tuebingen.de"
Journal Title:		Plant Cell
Year:		2015
Volume:		20150421
Issue:		4
Page Number:		1082 - 1097
DOI:		10.1105/tpc.114.135160
ISSN/ISBN:		1532-298X (Electronic) 1040-4651 (Print) 1040-4651 (Linking)
Abstract:		"Diphosphorylated inositol polyphosphates, also referred to as inositol pyrophosphates, are important signaling molecules that regulate critical cellular activities in many eukaryotic organisms, such as membrane trafficking, telomere maintenance, ribosome biogenesis, and apoptosis. In mammals and fungi, two distinct classes of inositol phosphate kinases mediate biosynthesis of inositol pyrophosphates: Kcs1/IP6K- and Vip1/PPIP5K-like proteins. Here, we report that PPIP5K homologs are widely distributed in plants and that Arabidopsis thaliana VIH1 and VIH2 are functional PPIP5K enzymes. We show a specific induction of inositol pyrophosphate InsP8 by jasmonate and demonstrate that steady state and jasmonate-induced pools of InsP8 in Arabidopsis seedlings depend on VIH2. We identify a role of VIH2 in regulating jasmonate perception and plant defenses against herbivorous insects and necrotrophic fungi. In silico docking experiments and radioligand binding-based reconstitution assays show high-affinity binding of inositol pyrophosphates to the F-box protein COI1-JAZ jasmonate coreceptor complex and suggest that coincidence detection of jasmonate and InsP8 by COI1-JAZ is a critical component in jasmonate-regulated defenses"
Keywords:		"Arabidopsis/genetics/*metabolism Arabidopsis Proteins/genetics/*metabolism Cyclopentanes/*metabolism Gene Expression Regulation, Plant/genetics/physiology Inositol Phosphates/*metabolism Oxylipins/*metabolism;"
Notes:		"MedlineLaha, Debabrata Johnen, Philipp Azevedo, Cristina Dynowski, Marek Weiss, Michael Capolicchio, Samanta Mao, Haibin Iven, Tim Steenbergen, Merel Freyer, Marc Gaugler, Philipp de Campos, Marilia K F Zheng, Ning Feussner, Ivo Jessen, Henning J Van Wees, Saskia C M Saiardi, Adolfo Schaaf, Gabriel eng R01CA107134/CA/NCI NIH HHS/ HHMI/Howard Hughes Medical Institute/ MC_UU_12018/4/MRC_/Medical Research Council/United Kingdom R01 CA107134/CA/NCI NIH HHS/ MC_U122680443/MRC_/Medical Research Council/United Kingdom Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't England 2015/04/23 Plant Cell. 2015 Apr; 27(4):1082-97. doi: 10.1105/tpc.114.135160. Epub 2015 Apr 21"