| Title: | "Physiological and Oxidative Stress Responses of Lettuce to Cleomside A: A Thiohydroximate, as a New Allelochemical from Cleome arabica L" |
| Author(s): | Ladhari A; Andolfi A; DellaGreca M; |
| Address: | "Laboratoire GREEN-TEAM (LR17AGR01), Institut National Agronomique de Tunisie (INAT), Universite de Carthage, 43 Avenue Charles Nicolle, Tunis 1082, Tunisia. Dipartimento di Scienze Chimiche, Universita Federico II, Complesso Universitario Monte S. Angelo, via Cintia, 4, 80126 Napoli, Italy. BAT Center-Interuniversity Center for Studies on Bioinspired Agro-Environmental Technology, University of Napoli 'Federico II', 80138 Naples, Italy" |
| DOI: | 10.3390/molecules25194461 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "The inclination toward natural products have led the onset for the discovery of new bioactive metabolites that could be targeted for specific therapeutic or agronomic applications. This study aimed to isolate bioactive compounds from Cleome arabica L., and subsequently determine the unexplored mechanism of action of the newly identified compounds on Lactuca sativa L. Chemical investigation of the ethyl acetate fraction of methanolic silique extract of C. arabica afforded seven secondary metabolites belonging to different classes such as flavonoids, triterpene, and a new thiohydroximate derivative, named cleomside A. Among phytotoxic assays, the growth of lettuce was totally inhibited by cleomside A compared to the other identified compounds. This effect was associated with the increased levels of electrolyte leakage, malondialdehyde, and hydrogen peroxide indicating disruption of membrane integrity and induction of oxidative stress. Activities of the antioxidant enzymes SOD, CAT, and APX were also elevated, thereby demonstrating the enhanced generation of reactive oxygen species upon identified allelochemical exposure. Thus, the changes caused by cleomside A described herein can contribute to better understanding the allelochemical actions of thiohydroximate and the potential use of these substances in the production of natural herbicides compared to the other identified flavonoids and triterpene" |
| Keywords: | Acetates/chemistry Antioxidants/pharmacology Biomarkers/analysis Carbon-13 Magnetic Resonance Spectroscopy Cleome/*chemistry Cluster Analysis Electrolytes/metabolism Hydrogen Peroxide/metabolism Lettuce/drug effects/enzymology/*physiology Mitotic Index Ox; |
| Notes: | "MedlineLadhari, Afef Andolfi, Anna DellaGreca, Marina eng Switzerland 2020/10/02 Molecules. 2020 Sep 28; 25(19):4461. doi: 10.3390/molecules25194461" |
