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Antibiotics (Basel)

Title:		Susceptibility Testing by Volatile Organic Compound Detection Direct from Positive Blood Cultures: A Proof-of-Principle Laboratory Study
Author(s):		Kuil SD; Hidad S; Schneeberger C; Singh P; Rhodes P; de Jong MD; Visser CE;
Address:		"Department of Medical Microbiology, Amsterdam Infection & Immunity Institute, Amsterdam UMC, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands. Specific Diagnostics, San Jose, CA 95134, USA"
Journal Title:		Antibiotics (Basel)
Year:		2022
Volume:		20220524
Issue:		6
Page Number:		-
DOI:		10.3390/antibiotics11060705
ISSN/ISBN:		2079-6382 (Print) 2079-6382 (Electronic) 2079-6382 (Linking)
Abstract:		"BACKGROUND: Bacteria produce volatile organic compounds (VOCs) during growth, which can be detected by colorimetric sensor arrays (CSAs). The SpecifAST((R)) system (Specific Diagnostics) employs this technique to enable antibiotic susceptibility testing (AST) directly from blood cultures without prior subculture of isolates. The aim of this study was to compare the SpecifAST((R)) AST results and analysis time to the VITEK((R))2 (bioMerieux) system. METHODS: In a 12-month single site prospective study, remnants of clinical positive monomicrobial blood cultures were combined with a series of antibiotic concentrations. Volatile emission was monitored at 37 degrees C via CSAs. Minimal Inhibitory Concentrations (MICs) of seven antimicrobial agents for Enterobacterales, Staphylococcus, and Enterococcus spp. were compared to VITEK((R))2 AST results. MICs were interpreted according to EUCAST clinical breakpoints. Performance was assessed by calculating agreement and discrepancy rates. RESULTS: In total, 96 positive blood cultures containing Enterobacterales, Staphylococcus, and Enterococcus spp. were tested (269 bug-drug combinations). The categorical agreement of the SpecifAST((R)) system compared to the VITEK((R))2 system was 100% and 91% for Gram-negatives and Gram-positives, respectively. Errors among Gram-positives were from coagulase-negative staphylococci. Overall results were available in 3.1 h (+/-0.9 h) after growth detection without the need for subculture steps. CONCLUSION: The AST results based on VOC detection are promising and warrant further evaluation in studies with a larger sample of bacterial species and antimicrobials"
Keywords:		blood cultures direct antimicrobial susceptibility testing phenotypic antimicrobial susceptibility testing rapid diagnostics volatile organic compounds;
Notes:		"PubMed-not-MEDLINEKuil, Sacha Danielle Hidad, Soemeja Schneeberger, Caroline Singh, Pragya Rhodes, Paul de Jong, Menno Douwe Visser, Caroline Elisabeth eng 2017-26/Academic Medical Center/ Switzerland 2022/06/25 Antibiotics (Basel). 2022 May 24; 11(6):705. doi: 10.3390/antibiotics11060705"