| Title: | Passive dosing: Assessing the toxicity of individual PAHs and recreated mixtures to the microalgae Raphidocelis subcapitata |
| Author(s): | Kreutzer A; Faetsch S; Heise S; Hollert H; Witt G; |
| Address: | "Department Evolutionary Ecology and Environmental Toxicology, Institute of Ecology, Evolution and Diversity, Faculty Biological Sciences, Goethe University Frankfurt, Frankfurt 60438, Germany; Department of Environmental Engineering, Faculty Life Sciences, Hamburg University of Applied Sciences, Hamburg 21033, Germany. Department of Biomedical Engineering, Faculty Life Sciences, Hamburg University of Applied Sciences, Hamburg 21033, Germany. Department Evolutionary Ecology and Environmental Toxicology, Institute of Ecology, Evolution and Diversity, Faculty Biological Sciences, Goethe University Frankfurt, Frankfurt 60438, Germany. Department of Environmental Engineering, Faculty Life Sciences, Hamburg University of Applied Sciences, Hamburg 21033, Germany. Electronic address: gesine.witt@haw-hamburg.de" |
| DOI: | 10.1016/j.aquatox.2022.106220 |
| ISSN/ISBN: | 1879-1514 (Electronic) 0166-445X (Linking) |
| Abstract: | "Risk assessment of hydrophobic organic compounds (HOCs) is difficult because maintaining a well-defined exposure during aquatic toxicity testing is challenging due to the limited water solubility and various loss processes such as volatilization, biodegradation and sorption. Passive dosing techniques help to overcome these challenges by providing a well-controlled and solvent-free exposure. In this study, the algal growth inhibition test (DIN EN ISO 8692) was converted into a miniaturized passive dosing setting. For this purpose, biocompatible O-rings were used as substance reservoirs and loaded with polycyclic aromatic hydrocarbons (PAHs). The growth inhibition of the microalgae Raphidocelis subcapitata induced by single PAHs (log K(OW) 3.24-5.91) was investigated. In addition, recreated PAH mixtures were tested representing field compositions of the pore water North Sea sediments. Some of the single PAHs revealed strong growth inhibiting effects on the algal growth, while the recreated mixture compositions had slightly lower effect on the growth inhibition in the highest concentrations. Overall, the toxicity of the PAHs generally increased with the maximum chemical activities (a(max)) of the PAHs and the inhibition data could be fitted with one maximum chemical activity response curve. Therefore, the miniaturized passive dosing approach appears as a promising practical and economical method that can be used for toxicity testing of the different trophic levels to improve comprehensive risk assessment" |
| Keywords: | "*Microalgae *Polycyclic Aromatic Hydrocarbons/chemistry/toxicity Toxicity Tests/methods Water/chemistry *Water Pollutants, Chemical/toxicity Algal growth inhibition Mixture toxicity Passive dosing Polycyclic aromatic hydrocarbons R.subcapitata;" |
| Notes: | "MedlineKreutzer, Anne Faetsch, Sonja Heise, Susanne Hollert, Henner Witt, Gesine eng Netherlands 2022/07/02 Aquat Toxicol. 2022 Aug; 249:106220. doi: 10.1016/j.aquatox.2022.106220. Epub 2022 Jun 10" |
