| Title: | On the cytotoxicity of some microbial volatile organic compounds as studied in the human lung cell line A549 |
| Address: | "Institute of Occupational, Social and Environmental Medicine, University of Ulm, Germany. ludwika.kreja@medizin.uni-ulm.de" |
| DOI: | 10.1016/s0045-6535(02)00159-5 |
| ISSN/ISBN: | 0045-6535 (Print) 0045-6535 (Linking) |
| Abstract: | "The cytotoxicity of 13 microbial volatile organic compounds (MVOC) was studied using a human lung carcinoma epithelial cell line A549 in a colony formation assay and two colorimetric assays: the microculture tetrazolium assay (MTT assay) and the cellular protein assay (methylene blue-MB assay). For comparison, two known cytotoxic substances: the non-volatile mycotoxin gliotoxin and the mono-functional alkylating agent methyl methanesulfonate (MMS) were studied. Concentration-response curves for each agent were established and the IC50 value (concentration resulting in 50% inhibition of colony growth or absorbance) was estimated. There are differences in toxicity levels between the MVOC tested and gliotoxin and MMS. The most toxic MVOC was 1-decanol which was as effective as MMS in all test systems. 1-decanol was about 10-fold more toxic than the other MVOC. All MVOC tested were more than 1000-fold less toxic than gliotoxin" |
| Keywords: | "Bacteria/*chemistry Carcinoma/drug therapy Colorimetry/methods Dose-Response Relationship, Drug Epithelial Cells/drug effects Fatty Alcohols/toxicity Gliotoxin/toxicity Humans Inhibitory Concentration 50 Lung Neoplasms/drug therapy Methyl Methanesulfonate;" |
| Notes: | "MedlineKreja, Ludwika Seidel, Hans-Joachim eng Research Support, Non-U.S. Gov't England 2002/09/24 Chemosphere. 2002 Oct; 49(1):105-10. doi: 10.1016/s0045-6535(02)00159-5" |
