| Title: | Specific Mycoparasite-Fusarium Graminearum Molecular Signatures in Germinating Seeds Disabled Fusarium Head Blight Pathogen's Infection |
| Author(s): | Kim SH; Lahlali R; Karunakaran C; Vujanovic V; |
| Address: | "Department of Food and Bioproduct Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK S7N 5A8, Canada. Canadian Light Source, 44 Innovation Blvd, Saskatoon, SK S7N 2V3, Canada. Department of Plant Protection, Phytopathology Unit, Ecole Nationale d'Agriculture de Meknes, BP/S 40, Meknes 50001, Morocco" |
| ISSN/ISBN: | 1422-0067 (Electronic) 1422-0067 (Linking) |
| Abstract: | "Advances in Infrared (IR) spectroscopies have entered a new era of research with applications in phytobiome, plant microbiome and health. Fusarium graminearum 3-ADON is the most aggressive mycotoxigenic chemotype causing Fusarium head blight (FHB) in cereals; while Sphaerodes mycoparasitica is the specific Fusarium mycoparasite with biotrophic lifestyle discovered in cereal seeds and roots. Fourier transform infrared (FTIR) spectroscopy analyses depicted shifts in the spectral peaks related to mycoparasitism mainly within the region of proteins, lipids, also indicating a link between carbohydrates and protein regions, involving potential phenolic compounds. Especially, S. mycoparasitica contributes to significant changes in lipid region 3050-2800 cm(-1), while in the protein region, an increasing trend was observed for the peaks 1655-1638 cm(-1) (amide I) and 1549-1548 cm(-1) (amide II) with changes in indicative protein secondary structures. Besides, the peak extending on the region 1520-1500 cm(-1) insinuates a presence of aromatic compounds in presence of mycoparasite on the F. graminearum root sample. Monitoring shift in improved seed germination, fungus-fungus interface through scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM), and FTIR molecular signatures combined with principal component analysis (PCA) proved useful tools to detect an early mycoparasitism as a vital asset of the preventive biocontrol strategy against plant pathogens" |
| Keywords: | "Ascomycota/*physiology Biological Control Agents Fungal Proteins/genetics/*metabolism Fusarium/*pathogenicity Gene Expression Regulation, Fungal *Germination Plant Diseases/microbiology/*prevention & control Seeds/*growth & development/microbiology Volati;" |
| Notes: | "MedlineKim, Seon Hwa Lahlali, Rachid Karunakaran, Chithra Vujanovic, Vladimir eng RGPIN-2017-05286/Natural Sciences and Engineering Research Council of Canada/ Switzerland 2021/03/07 Int J Mol Sci. 2021 Feb 28; 22(5):2461. doi: 10.3390/ijms22052461" |
