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Biochem Biophys Res Commun

Title:		Posttranscriptional regulation of the karyogamy gene by Kem1p/Xrn1p exoribonuclease and Rok1p RNA helicase of Saccharomyces cerevisiae
Author(s):		Kim J; Jeon S; Yang YS; Kim J;
Address:		"Department of Microbiology, School of Bioscience and Biotechnology, Chungnam National University, Daejeon 305-764, Republic of Korea. jmkim@cnu.ac.kr"
Journal Title:		Biochem Biophys Res Commun
Year:		2004
Volume:		321
Issue:		4
Page Number:		1032 - 1039
DOI:		10.1016/j.bbrc.2004.07.065
ISSN/ISBN:		0006-291X (Print) 0006-291X (Linking)
Abstract:		"The major biochemical activities ascribed to Kem1p/Xrn1p of Saccharomyces cerevisiae are 5'-3' exoribonuclease functioning in RNA turnover and a microtubule-binding protein. Mutational analysis has shown that Kem1p/Xrn1p participates in microtubule-related functions such as nuclear fusion (karyogamy) during mating, chromosome transmission, and spindle pole body duplication. Here, evidence is presented that Kem1p plays a specific role in nuclear fusion by affecting, at the posttranscriptional level, the pheromone induction of the karyogamy-specific transcription factor Kar4p and the expression of Rok1p, a putative RNA helicase. We found that Rok1p itself also affects the pheromone induction of Kar4p and thereby participates in nuclear fusion. Analysis of the active-site mutations, xrn1-D206A or D208A, shows that nuclear fusion as well as the Rok1p synthesis do not require the exoribonuclease activity of Kem1p. Our data provide an important insight into the gene-specific regulatory function mediated by the general RNA-modulating enzymes"
Keywords:		"Base Sequence Benomyl/pharmacology DEAD-box RNA Helicases DNA, Fungal/genetics DNA-Binding Proteins/genetics/metabolism Exoribonucleases/genetics/*metabolism Fungicides, Industrial/pharmacology *Genes, Fungal Meiosis/drug effects/genetics Mutation Phenoty;"
Notes:		"MedlineKim, Jaehee Jeon, Soonmee Yang, Yun-Seok Kim, Jinmi eng Research Support, Non-U.S. Gov't 2004/09/11 Biochem Biophys Res Commun. 2004 Sep 3; 321(4):1032-9. doi: 10.1016/j.bbrc.2004.07.065"