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Int J Mol Sci

Title:		"A Cross-Sectional Study on 3-(2-Deoxy-beta-D-Erythro-Pentafuranosyl)Pyrimido[1,2-alpha]Purin-10(3H)-One Deoxyguanosine Adducts among Woodworkers in Tuscany, Italy"
Author(s):		Cellai F; Capacci F; Sgarrella C; Poli C; Arena L; Tofani L; Giese RW; Peluso M;
Address:		"Cancer Factor Risk Branch, Regional Cancer Prevention Laboratory, Institute for Cancer Research, Prevention and Clinical Network (ISPRO), 50139 Florence, Italy. f.cellai@ispro.toscana.it. Functional Unit for Prevention, Health and Safety in the Workplace, ASL10, 50139 Florence, Italy. fabio.capacci@uslcentro.toscana.it. Functional Unit for Prevention, Health and Safety in the Workplace, ASL10, 50139 Florence, Italy. carla.sgarrella@uslcentro.toscana.it. Department of Prevention, Azienda USL Toscana Centro, 50139 Florence, Italy. carla.poli@uslcentro.toscana.it. Department of Prevention, Azienda USL Toscana Centro, 50139 Florence, Italy. luciano.arena@uslcentro.toscana.it. Department of Neurosciences, Psychology, Drug Research and Child Health, University of Florence, 50139 Florence, Italy. lorenzo120787@gmail.com. Bouve College of Health Sciences, Barnett Institute, Northeastern University, Northeastern University, Boston, MA 02115, USA. r.giese@northeastern.edu. Cancer Factor Risk Branch, Regional Cancer Prevention Laboratory, Institute for Cancer Research, Prevention and Clinical Network (ISPRO), 50139 Florence, Italy. m.peluso@ispro.toscana.it"
Journal Title:		Int J Mol Sci
Year:		2019
Volume:		20190605
Issue:		11
Page Number:		-
DOI:		10.3390/ijms20112763
ISSN/ISBN:		1422-0067 (Electronic) 1422-0067 (Linking)
Abstract:		"Occupational exposure to wood dust has been estimated to affect 3.6 million workers within the European Union (EU). The most serious health effect caused by wood dust is the nasal and sinonasal cancer (SNC), which has been observed predominantly among woodworkers. Free radicals produced by inflammatory reactions as a consequence of wood dust could play a major role in SNC development. Therefore, we investigated the association between wood dust and oxidative DNA damage in the cells of nasal epithelia, the target site of SNC. We have analyzed oxidative DNA damage by determining the levels of 3-(2-deoxy-beta-D-erythro-pentafuranosyl)pyrimido[1,2-alpha]purin-10(3H)-one deoxyguanosine (M(1)dG), a major-peroxidation-derived DNA adduct and a biomarker of cancer risk in 136 woodworkers compared to 87 controls in Tuscany, Italy. We then examined the association of M(1)dG with co-exposure to volatile organic compounds (VOCs), exposure length, and urinary 15-F(2t) isoprostane (15-F(2t)-IsoP), a biomarker of oxidant status. Wood dust at the workplace was estimated by the Information System for Recording Occupational Exposures to Carcinogens. M(1)dG was measured using (32)P-postlabeling and mass spectrometry. 15-F(2t)-IsoP was analyzed using ELISA. Results show a significant excess of M(1)dG in the woodworkers exposed to average levels of 1.48 mg/m(3) relative to the controls. The overall mean ratio (MR) between the woodworkers and the controls was 1.28 (95% C.I. 1.03-1.58). After stratification for smoking habits and occupational status (exposure to wood dust alone and co-exposure to VOCs), the association of M(1)dG with wood dust (alone) was even greater in non-smokers workers, MR of 1.43 (95% C.I. 1.09-1.87). Conversely, not consistent results were found in ex-smokers and current smokers. M(1)dG was significantly associated with co-exposure to VOCs, MR of 1.95 (95% C.I. 1.46-2.61), and occupational history, MR of 2.47 (95% C.I. 1.67-3.62). Next, the frequency of M(1)dG was significantly correlated to the urinary excretion of 15-F(2t)-IsoP, regression coefficient (beta) = 0.442 +/- 0.172 (SE). Consistent with the hypothesis of a genotoxic mechanism, we observed an enhanced frequency of M(1)dG adducts in woodworkers, even at the external levels below the regulatory limit. Our data implement the understanding of SNC and could be useful for the management of the adverse effects caused by this carcinogen"
Keywords:		Cross-Sectional Studies DNA Adducts/*metabolism Deoxyguanosine/*metabolism Dinoprost/analogs & derivatives/metabolism Dust/analysis Humans Italy Male Middle Aged *Occupational Exposure Pyrimidinones/*metabolism Reference Standards *Wood M1dG VOCs nasal ep;neuroscience;
Notes:		"MedlineCellai, Filippo Capacci, Fabio Sgarrella, Carla Poli, Carla Arena, Luciano Tofani, Lorenzo Giese, Roger W Peluso, Marco eng na/Regione Toscana/ Switzerland 2019/06/15 Int J Mol Sci. 2019 Jun 5; 20(11):2763. doi: 10.3390/ijms20112763"