| Title: | "Toxic, Radical Scavenging, and Antifungal Activity of Rhododendron tomentosum H. Essential Oils" |
| Author(s): | Judzentiene A; Budiene J; Svediene J; Garjonyte R; |
| Address: | "State Research Institute Center for Physical Sciences and Technology, Department of Organic Chemistry; Sauletekio Ave. 3, LT-10222 Vilnius, Lithuania. Nature Research Centre, Laboratory of Biodeterioration Research; Akademijos 2, LT-08412 Vilnius, Lithuania" |
| DOI: | 10.3390/molecules25071676 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "The chemical composition of eight (seven shoot and one inflorescence) essential oils (EOs) of Rh. tomentosum H. plants growing in Eastern Lithuania is reported. The plant material was collected during different phases of vegetation (from April to October). The oils were obtained by hydrodistillation from air-dried aerial parts (leaves and inflorescences). In total, up to 70 compounds were identified by GC-MS and GC (flame-ionization detector, FID); they comprised 91.0 +/- 4.7%-96.2 +/- 3.1% of the oil content. Sesquiterpene hydrocarbons (54.1 +/- 1.5%-76.1 +/- 4.5%) were found to be the main fraction. The major compounds were palustrol (24.6 +/- 2.6%-33.5 +/- 4.4%) and ledol (18.0 +/- 2.9%-29.0 +/- 5.0%). Ascaridol isomers (7.0 +/- 2.4%-14.0 +/- 2.4% in three oils), myrcene (7.2 +/- 0.3% and 10.1 +/- 1.3%), lepalol (3.3 +/- 0.3% and 7.9 +/- 3.0%), and cyclocolorenone isomers (4.1 +/- 2.5%) were determined as the third main constituents. The toxic activity of marsh rosemary inflorescence and shoot oils samples was evaluated using a brine shrimp (Artemia sp.) bioassay. LC(50) average values (11.23-20.50 microg/mL) obtained after 24 h of exposure revealed that the oils were notably toxic. The oil obtained from shoots gathered in September during the seed-ripening stage and containing appreciable amounts of palustrol (26.0 +/- 2.5%), ledol (21.5 +/- 4.0%), and ascaridol (7.0 +/- 2.4%) showed the highest toxic activity. Radical scavenging activity of Rh. tomentosum EOs depended on the plant vegetation stage. The highest activities were obtained for EOs isolated from young shoots collected in June (48.19 +/- 0.1 and 19.89 +/- 0.3 mmol/L TROLOX (6-hydroxy-2,5,7,8-tetra-methylchromane-2-carboxylic acid) equivalent obtained by, respectively, ABTS(*)(+) (2,2'-amino-bis(ethylbenzothiazoline-6-sulfonic acid) diammonium salt) and DPPH(*)(2,2-diphenyl-1-picrylhydrazyl) assays). Agar disc diffusion assay against pathogenic yeast Candida parapsilosis revealed the potential antifungal activity of EOs. An alternative investigation of antifungal activity employed mediated amperometry at yeast Saccharomyces cerevisiae-modified electrodes. The subjection of yeast cells to vapors of EO resulted in a three to four-fold increase of electrode responses due to the disruption of yeast cell membranes" |
| Keywords: | "Antifungal Agents/chemistry/*pharmacology Antioxidants/chemistry/pharmacology Ericaceae/chemistry Oils, Volatile/chemistry/*pharmacology Rhododendron/*chemistry Ericaceae Rhododendron tomentosum H.amperometry antifungal activity antioxidant tests essenti;" |
| Notes: | "MedlineJudzentiene, Asta Budiene, Jurga Svediene, Jurgita Garjonyte, Rasa eng Switzerland 2020/04/09 Molecules. 2020 Apr 5; 25(7):1676. doi: 10.3390/molecules25071676" |
