| Title: | "Occupational Exposure in Industrial Painters: Sensitive and Noninvasive Biomarkers to Evaluate Early Cytotoxicity, Genotoxicity and Oxidative Stress" |
| Author(s): | Cavallo D; Ursini CL; Fresegna AM; Ciervo A; Maiello R; Buresti G; Paci E; Pigini D; Gherardi M; Carbonari D; Sisto R; Tranfo G; Iavicoli S; |
| Address: | "Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers' Compensation Authority-INAIL, Monte Porzio Catone, 00078 Rome, Italy" |
| Journal Title: | Int J Environ Res Public Health |
| ISSN/ISBN: | 1660-4601 (Electronic) 1661-7827 (Print) 1660-4601 (Linking) |
| Abstract: | "This study aimed to identify sensitive and noninvasive biomarkers of early cyto-genotoxic, oxidative and inflammatory effects for exposure to volatile organic compounds (VOCs) in shipyard painters. On 17 (11 spray and 6 roller) painters (previously characterized for VOCs exposure to toluene, xylenes, ethylbenzene, ethyl acetate) and on 18 controls, we performed buccal micronucleus cytome (BMCyt) assay; Fpg-comet assay on lymphocytes; detection of urinary 8-oxoGua (8-oxo-7,8-dihydroguanine), 8-oxodGuo (8-oxo-7,8-dihydro-2'-deoxyguanosine) and 8-oxoGuo (8-oxo-7,8-dihydroguanosine), and cytokines release on serum. We found induction of cyto-genotoxicity by BMCyt assay and inflammatory effects (IL-6 and TNFalpha) in roller painters exposed to lower VOC concentrations than spray painters. In contrast, in both worker groups, we found direct and oxidative DNA damage by comet assay (with slightly higher oxidative DNA damage in roller) and significant increase of 8-oxoGuo and decrease of 8-oxodGuo and 8-oxoGua in respect to controls. The cyto-genotoxicity observed only on buccal cells of roller painters could be related to the task's specificity and the different used protective equipment. Although limited by the small number of subjects, the study shows the usefulness of all the used biomarkers in the risk assessment of painters workers exposed to complex mixtures" |
| Keywords: | Biomarkers/metabolism Comet Assay DNA Damage Humans *Mouth Mucosa/metabolism *Occupational Exposure/adverse effects/analysis Oxidative Stress BMCyt assay Fpg comet assay biological monitoring occupational VOCs exposure oxidative stress biomarkers painters; |
| Notes: | "MedlineCavallo, Delia Ursini, Cinzia Lucia Fresegna, Anna Maria Ciervo, Aureliano Maiello, Raffaele Buresti, Giuliana Paci, Enrico Pigini, Daniela Gherardi, Monica Carbonari, Damiano Sisto, Renata Tranfo, Giovanna Iavicoli, Sergio eng Switzerland 2021/05/01 Int J Environ Res Public Health. 2021 Apr 27; 18(9):4645. doi: 10.3390/ijerph18094645" |
