| Title: | Olfactory receptor OR52N2 for PGE(2) in mediation of guppy courtship behaviors |
| Author(s): | Jing X; Lyu L; Gong Y; Wen H; Li Y; Wang X; Li J; Yao Y; Zuo C; Xie S; Yan S; Qi X; |
| Address: | "Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, China. Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, China. Electronic address: qx@ouc.edu.cn" |
| DOI: | 10.1016/j.ijbiomac.2023.124518 |
| ISSN/ISBN: | 1879-0003 (Electronic) 0141-8130 (Linking) |
| Abstract: | "Prostaglandins (PGs) are a type of physiologically active unsaturated fatty acids. As an important sex pheromone, PGs play a vital role in regulating the reproductive behaviors of species by mediating nerve and endocrine responses. In this study, guppy (Poecilia reticulate) was used as the model specie to detect the function of PGE(2) in inducing the onset of courtship behaviors. Our results showed that adding PGE(2) into the water environment could activate the courtship behavior of male guppy, indicating that the peripheral olfactory system mediated the PGE(2) function. Thereafter, the open reading frame (ORF) of olfactory receptor or52n2 was cloned, which was 936 bp in length, coding 311 amino acids. As a typical G protein-coupled receptor, OR52N2 had a conservative seven alpha-helix transmembrane domains. To confirm the regulatory relationship between OR52N2 and PGE(2), dual-luciferase reporter assay was employed to verify the activation of downstream CREB signaling pathways. Results showed that PGE(2) significantly enhanced CRE promoter activity in or52n2 ORF transient transfected HEK-293 T cells. Finally, localization of or52n2 mRNA were observed in ciliated receptor cells of the olfactory epithelium using in situ hybridization. Our results provide a novel insight into sex pheromone signaling transduction in reproductive behavior" |
| Keywords: | "Humans Animals Male *Receptors, Odorant/metabolism *Poecilia/metabolism Dinoprostone *Sex Attractants Courtship HEK293 Cells Courtship behavior Olfactory receptor or52n2 Poecilia reticulata Prostaglandin E(2);" |
| Notes: | "MedlineJing, Xiao Lyu, Likang Gong, Yu Wen, Haishen Li, Yun Wang, Xiaojie Li, Jianshuang Yao, Yijia Zuo, Chenpeng Xie, Songyang Yan, Shaojing Qi, Xin eng Netherlands 2023/04/24 Int J Biol Macromol. 2023 Jun 30; 241:124518. doi: 10.1016/j.ijbiomac.2023.124518. Epub 2023 Apr 21" |
