| Title: | OsPHR2 modulates phosphate starvation-induced OsMYC2 signalling and resistance to Xanthomonas oryzae pv. oryzae |
| Author(s): | Kong Y; Wang G; Chen X; Li L; Zhang X; Chen S; He Y; Hong G; |
| Address: | "State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China. College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, China. National Key Laboratory of Plant Molecular Genetics and National Plant Gene Research Center, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Shanghai, China. Institute of Plant Protection, Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Jiangsu Academy of Agricultural Sciences, Nanjing, China" |
| ISSN/ISBN: | 1365-3040 (Electronic) 0140-7791 (Linking) |
| Abstract: | "Phosphate (Pi) and MYC2-mediated jasmonate (JA) pathway play critical roles in plant growth and development. In particular, crosstalk between JA and Pi starvation signalling has been reported to mediate insect herbivory resistance in dicot plants. However, its roles and mechanism in monocot-bacterial defense systems remain obscure. Here, we report that Pi starvation in rice activates the OsMYC2 signalling and enhances resistance to Xanthomonas oryzae pv. oryzae (Xoo) infection. The direct regulation of OsPHR2 on the OsMYC2 promoter was confirmed by yeast one-hybrid, electrophoretic mobility shift, dual-luciferase and chromatin immunoprecipitation assays. Molecular analyses and infection studies using OsPHR2-Ov1 and phr2 mutants further demonstrated that OsPHR2 enhances antibacterial resistance via transcriptional regulation of OsMYC2 expression, indicating a positive role of OsPHR2-OsMYC2 crosstalk in modulating the OsMYC2 signalling and Xoo infection. Genetic analysis and infection assays using myc2 mutants revealed that Pi starvation-induced OsMYC2 signalling activation and consequent Xoo resistance depends on the regulation of OsMYC2. Together, these results reveal a clear interlink between Pi starvation- and OsMYC2- signalling in monocot plants, and provide new insight into how plants balance growth and defence by integrating nutrient deficiency and phytohormone signalling. We highlighted a molecular link connecting OsMYC2-mediated JA pathway and phosphate starvation signalling in monocot plant. We demonstrated that phosphate starvation promoted OsMYC2 signalling to enhance rice defence to bacterial blight via transcriptional regulation of OsPHR2 on OsMYC2" |
| Keywords: | Cyclopentanes/metabolism Disease Resistance/genetics Oryza/*genetics/metabolism Oxylipins/metabolism Phosphorus/*deficiency Plant Diseases/*genetics/microbiology Plant Proteins/*genetics/metabolism Signal Transduction/genetics Xanthomonas/*physiology OsMY; |
| Notes: | "MedlineKong, Yaze Wang, Gang Chen, Xian Li, Linying Zhang, Xueying Chen, Sangtian He, Yuqing Hong, Gaojie eng National Natural Science Foundation of China: 31670291, 31800249/ Zhejiang Provincial Natural Science Foundation of China: LQ18C140004/ 2010DS700124-ZZ2017/State Key Laboratory for Managing Biotic andChemical Threats to the Quality and Safety of Agro-products/ Research Support, Non-U.S. Gov't 2021/05/04 Plant Cell Environ. 2021 Oct; 44(10):3432-3444. doi: 10.1111/pce.14078. Epub 2021 Aug 19" |
