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Genes Dev

Title:		Developmentally induced changes in transcriptional program alter spatial organization across chromosomes
Author(s):		Casolari JM; Brown CR; Drubin DA; Rando OJ; Silver PA;
Address:		"Department of Systems Biology, Harvard Medical School and The Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA"
Journal Title:		Genes Dev
Year:		2005
Volume:		19
Issue:		10
Page Number:		1188 - 1198
DOI:		10.1101/gad.1307205
ISSN/ISBN:		0890-9369 (Print) 0890-9369 (Linking)
Abstract:		"Although the spatial location of genes within the nucleus has been implicated in their transcriptional status, little is known about the dynamics of gene location that accompany large-scale changes in gene expression. The mating of haploid yeast Saccharomyces cerevisiae is accompanied by a large-scale change in transcription and developmental program. We examined changes in nuclear organization that accompany stimulus by the mating pheromone alpha factor and found that most alpha-factor-induced genes become associated with components of the nuclear envelope. The myosin-like protein Mlp1, which has been implicated in mRNA export, was further shown to exhibit RNA dependence in its association with alpha-factor-induced genes. High-resolution mapping of association of chromosome III with Mlp1 revealed alpha-factor-dependent determinants of nuclear pore association, including origins of replication, specific intergenic regions, and the 3' ends of transcriptionally activated genes. Taken together, these results reveal RNA- and DNA-dependent determinants of nuclear organization as well as a detailed picture of how an entire chromosome alters its spatial conformation in response to a developmental cue"
Keywords:		"Chromosomes, Fungal/*physiology DNA, Intergenic/metabolism Gene Expression Regulation, Fungal/genetics/*physiology Nuclear Pore/metabolism Nuclear Proteins/metabolism Protein Precursors/pharmacology RNA, Messenger/metabolism RNA-Binding Proteins Replicati;"
Notes:		"MedlineCasolari, Jason M Brown, Christopher R Drubin, David A Rando, Oliver J Silver, Pamela A eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 2005/05/21 Genes Dev. 2005 May 15; 19(10):1188-98. doi: 10.1101/gad.1307205"