| Title: | The Rice Transcription Factor WRKY53 Suppresses Herbivore-Induced Defenses by Acting as a Negative Feedback Modulator of Mitogen-Activated Protein Kinase Activity |
| Author(s): | Hu L; Ye M; Li R; Zhang T; Zhou G; Wang Q; Lu J; Lou Y; |
| Address: | "State Key Laboratory of Rice Biology, Institute of Insect Science, Zhejiang University, Hangzhou 310058, China (L.H., M.Y., R.L., T.Z., G.Z., Q.W., J.L., Y.L.); andDepartment of Plant Protection, Key Laboratory for Quality Improvement of Agriculture Products of Zhejiang Province, Zhejiang Agriculture and Forestry University, Lin'an 311300, China (G.Z.). State Key Laboratory of Rice Biology, Institute of Insect Science, Zhejiang University, Hangzhou 310058, China (L.H., M.Y., R.L., T.Z., G.Z., Q.W., J.L., Y.L.); andDepartment of Plant Protection, Key Laboratory for Quality Improvement of Agriculture Products of Zhejiang Province, Zhejiang Agriculture and Forestry University, Lin'an 311300, China (G.Z.) yglou@zju.edu.cn" |
| ISSN/ISBN: | 1532-2548 (Electronic) 0032-0889 (Print) 0032-0889 (Linking) |
| Abstract: | "The mechanisms by which herbivore-attacked plants activate their defenses are well studied. By contrast, little is known about the regulatory mechanisms that allow them to control their defensive investment and avoid a defensive overshoot. We characterized a rice (Oryza sativa) WRKY gene, OsWRKY53, whose expression is rapidly induced upon wounding and induced in a delayed fashion upon attack by the striped stem borer (SSB) Chilo suppressalis. The transcript levels of OsWRKY53 are independent of endogenous jasmonic acid but positively regulated by the mitogen-activated protein kinases OsMPK3/OsMPK6. OsWRKY53 physically interacts with OsMPK3/OsMPK6 and suppresses their activity in vitro. By consequence, it modulates the expression of defensive, MPK-regulated WRKYs and thereby reduces jasmonic acid, jasmonoyl-isoleucine, and ethylene induction. This phytohormonal reconfiguration is associated with a reduction in trypsin protease inhibitor activity and improved SSB performance. OsWRKY53 is also shown to be a negative regulator of plant growth. Taken together, these results show that OsWRKY53 functions as a negative feedback modulator of MPK3/MPK6 and thereby acts as an early suppressor of induced defenses. OsWRKY53 therefore enables rice plants to control the magnitude of their defensive investment during early signaling" |
| Keywords: | "Amino Acid Sequence Animals Base Sequence Cyclopentanes/metabolism Disease Resistance/genetics Feedback, Physiological/physiology Gene Expression Regulation, Plant Herbivory/*physiology Host-Parasite Interactions Immunoblotting Mitogen-Activated Protein K;" |
| Notes: | "MedlineHu, Lingfei Ye, Meng Li, Ran Zhang, Tongfang Zhou, Guoxin Wang, Qi Lu, Jing Lou, Yonggen eng Research Support, Non-U.S. Gov't 2015/10/11 Plant Physiol. 2015 Dec; 169(4):2907-21. doi: 10.1104/pp.15.01090. Epub 2015 Oct 9" |
