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J Food Sci

Title:		Potential of Glycosidase from Non-Saccharomyces Isolates for Enhancement of Wine Aroma
Author(s):		Hu K; Qin Y; Tao YS; Zhu XL; Peng CT; Ullah N;
Address:		"College of Enology, Northwest A&F Univ, Yangling, Shaanxi, 712100, China. Shaanxi Engineering Research Center for Viti-Viniculture, Yangling, Shaanxi, 712100, China. Dept. of Human Nutrition, The Univ. of Agriculture Peshawar, 25000, Pakistan"
Journal Title:		J Food Sci
Year:		2016
Volume:		20160308
Issue:		4
Page Number:		M935 - M943
DOI:		10.1111/1750-3841.13253
ISSN/ISBN:		1750-3841 (Electronic) 0022-1147 (Linking)
Abstract:		"The aim of this work was to rapidly screen indigenous yeasts with high levels of beta-glucosidase activity and assess the potential of glycosidase extracts for aroma enhancement in winemaking. A semiquantitative colorimetric assay was applied using 96-well plates to screen yeasts from 3 different regions of China. Isolates with high beta-glucosidase activity were confirmed by the commonly used pNP assay. Among 493 non-Saccharomyces isolates belonging to 8 generas, 3 isolates were selected for their high levels of beta-glucosidase activity and were identified as Hanseniaspora uvarum, Pichia membranifaciens, and Rhodotorula mucilaginosa by sequence analysis of the 26S rDNA D1/D2 domain. beta-Glucosidase in the glycosidase extract from H. uvarum strain showed the highest activity in winemaking conditions among the selected isolates. For aroma enhancement in winemaking, the glycosidase extract from H. uvarum strain exhibited catalytic specificity for aromatic glycosides of C13 -norisoprenoids and some terpenes, enhancing fresh floral, sweet, berry, and nutty aroma characteristics in wine"
Keywords:		China *Fermentation Glycoside Hydrolases/metabolism Glycosides/analysis Hanseniaspora/*enzymology Humans Norisoprenoids/analysis Odorants/*analysis Pichia/*enzymology Rhodotorula/*enzymology Saccharomyces/metabolism Terpenes/analysis Wine/*analysis Yeasts;
Notes:		"MedlineHu, Kai Qin, Yi Tao, Yong-Sheng Zhu, Xiao-Lin Peng, Chuan-Tao Ullah, Niamat eng Research Support, Non-U.S. Gov't 2016/03/10 J Food Sci. 2016 Apr; 81(4):M935-43. doi: 10.1111/1750-3841.13253. Epub 2016 Mar 8"