| Title: | Towards the Identification of Antibiotic-Resistant Bacteria Causing Urinary Tract Infections Using Volatile Organic Compounds Analysis-A Pilot Study |
| Author(s): | Hewett K; Drabinska N; White P; Avison MB; Persad R; Ratcliffe N; Costello BL; |
| Address: | "Department of Applied Sciences, Faculty of Health and Applied Sciences, University of the West of England, Coldharbour Lane, Bristol BS16 1QY, UK. Department of Chemistry and Biodynamics of Food, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, 10-748 Olsztyn, Poland. Applied Statistics Group, Department of Engineering, Design and Mathematics, Faculty of Environment and Technology, University of the West of England, Bristol BS16 1QY, UK. School of Cellular & Molecular Medicine, Faculty of Life Sciences, University of Bristol, Bristol BS8 1TD, UK. Bristol Royal Infirmary and Bristol Urological Institute, Southmead Hospital, Bristol BS10 5BN, UK" |
| DOI: | 10.3390/antibiotics9110797 |
| ISSN/ISBN: | 2079-6382 (Print) 2079-6382 (Electronic) 2079-6382 (Linking) |
| Abstract: | "Antibiotic resistance is an unprecedented threat to modern medicine. The analysis of volatile organic compounds (VOCs) from bacteria potentially offers a rapid way to determine antibiotic susceptibility in bacteria. This study aimed to find the optimal conditions to obtain the maximum number of VOCs detected which next allowed the assessment of differences in VOC profiles between susceptible and resistant isolates of Escherichia coli causing urinary tract infections. The analysis of VOCs in the headspace above the bacterial cultures allowed the distinguishing of resistant and susceptible bacteria based on the abundance of six VOCs with 85.7% overall accuracy. The results of this preliminary study are promising, and with development could lead to a practical, faster diagnostic method for use in routine microbiology" |
| Keywords: | E.coli antibiotic resistance bacteria gas chromatography-mass spectrometry metabolite profiles susceptibility urinary tract infection volatile; |
| Notes: | "PubMed-not-MEDLINEHewett, Keith Drabinska, Natalia White, Paul Avison, Matthew B Persad, Raj Ratcliffe, Norman Costello, Ben de Lacy eng -/National Institute for Health Research (NIHR) Research Capability Funding (RCF), Avon Primary Care Research Collaborative/ Switzerland 2020/11/15 Antibiotics (Basel). 2020 Nov 11; 9(11):797. doi: 10.3390/antibiotics9110797" |
