| Title: | Technique for the identification of osmophores in flowers of herbarium material (TIOFH) |
| Address: | "Laboratorio de Morfologia Comparada de Espermatofitas (LAMCE), Facultad de Ciencias Agrarias y Forestales, UNLP, La Plata, Argentina. Division Plantas Vasculares, Museo de La Plata, Facultad de Ciencias Naturales y Museo, UNLP, La Plata, Argentina. katinas@fcnym.unlp.edu.ar" |
| DOI: | 10.1007/s00709-019-01398-8 |
| ISSN/ISBN: | 1615-6102 (Electronic) 0033-183X (Linking) |
| Abstract: | "The histochemical studies that search for osmophores differ in the protocol they follow for fixation and discoloration of the samples, and also in the type and number of stains utilized. Despite these differences, all the studies have one point in common: the use of fresh material either collected directly in the field or cultivated in botanical gardens and greenhouses. This is an obvious limitation for the studies of osmophores. Flower parts of herbarium specimens of different dates of collection and different plant families were exposed to variable times and percentages of discoloration agents and under different stains for finding if it is possible to positively test osmophores in this type of material. We obtained positive results discoloring the samples with ethyl alcohol 96 degrees and sodium hypochlorite, and staining with Lugol, Oil Red O, and Neutral Red (TIOFH). A protocol (TIOFH3) for osmophore testing that combines these three stains into a single procedure is proposed" |
| Keywords: | Flowers/*chemistry Odorants/*analysis Floral osmophores Herbarium material Histochemical technique Protocol; |
| Notes: | "MedlineHernandez, Marcelo P Katinas, Liliana eng PIP 0729/Consejo Nacional de Investigaciones Cientificas y Tecnicas/ PICT 2012-1683/Agencia Nacional de Promocion Cientifica y Tecnologica/ Austria 2019/06/12 Protoplasma. 2019 Nov; 256(6):1753-1765. doi: 10.1007/s00709-019-01398-8. Epub 2019 Jun 11" |
