| Title: | Electronic nicotine delivery systems exhibit reduced bronchial epithelial cells toxicity compared to cigarette: the Replica Project |
| Author(s): | Caruso M; Emma R; Distefano A; Rust S; Poulas K; Zadjali F; Giordano A; Volarevic V; Mesiakaris K; Al Tobi M; Boffo S; Arsenijevic A; Zuccarello P; Giallongo C; Ferrante M; Polosa R; Li Volti G; Replica Project G; |
| Address: | "Department of Biomedical and Biotechnological Sciences, University of Catania, Via S. Sofia, 97, 95123, Catania, Italy. Center of Excellence for the Acceleration of Harm Reduction (CoEHAR), University of Catania, Via S. Sofia, 97, 95123, Catania, Italy. ECLAT Srl, spin-off of the University of Catania, Via S. Sofia 89, 95123, Catania, Italy. Institute for Research and Innovation, IRIS, Patras Science Park, Patras, Greece. Laboratory of Molecular Biology and Immunology, Department of Pharmacy, University of Patras, Patras, Greece. College of Medicine and Health Sciences, Department of Clinical Biochemistry, Sultan Qaboos University, P.C 123, P.O. Box 35, Khodh, Oman. Sbarro Institute for Cancer Research and Molecular Medicine, Department of Biology, College of Science and Technology, Temple University, Philadelphia, USA. Center for Molecular Medicine and Stem Cell Research, Department of Microbiology and Immunology, Faculty of Medical Sciences, University of Kragujevac, 69 Svetozara Markovica Street, 34000, Kragujevac, Serbia. Department of Medical, Surgical Sciences and Advanced Technologies 'G.F. Ingrassia', University of Catania, Via S. Sofia, 87, 95123, Catania, Italy. Department of Clinical and Experimental Medicine, University of Catania, Via S. Sofia, 97, 95123, Catania, Italy. Department of Biomedical and Biotechnological Sciences, University of Catania, Via S. Sofia, 97, 95123, Catania, Italy. livolti@unict.it. Center of Excellence for the Acceleration of Harm Reduction (CoEHAR), University of Catania, Via S. Sofia, 97, 95123, Catania, Italy. livolti@unict.it" |
| DOI: | 10.1038/s41598-021-03310-y |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Electronic nicotine delivery systems (ENDS) may reduce health risks associated with chronic exposure to smoke and their potential benefits have been the matter of intense scientific debate. We aimed to replicate three published studies on cytotoxic and inflammatory effects of cigarette smoke and ENDS aerosol in an independent multi-center ring study. We aimed to establish the reliability of results and the robustness of conclusions by replicating the authors' experimental protocols and further validating them with different techniques. Human bronchial epithelial cells (NCI-H292) were exposed to cigarette whole smoke and vapor phase and to aerosol from ENDS. We also assessed the inflammatory cytokines interleukin-6 and interleukin-8 and the remodeling mediator matrix metalloproteinase-1. We replicated cell viability results and confirmed that almost 80% of cytotoxic effects are due to volatile compounds in the vapor phase of smoke. Our findings substantiated the reduced cytotoxic effects of ENDS aerosol. However, our data on inflammatory and remodeling activity triggered by smoke differed significantly from those in the original reports. Taken together, independent data from multiple laboratories clearly demonstrated the reduced toxicity of ENDS products compared to cigarettes" |
| Keywords: | Aerosols/adverse effects/chemistry Bronchi/cytology Cell Survival/drug effects Cytokines/metabolism Electronic Nicotine Delivery Systems Epithelial Cells Humans Inhibitory Concentration 50 Nicotine/*adverse effects Smoke/*adverse effects Tobacco/*adverse; |
| Notes: | "MedlineCaruso, Massimo Emma, Rosalia Distefano, Alfio Rust, Sonja Poulas, Konstantinos Zadjali, Fahad Giordano, Antonio Volarevic, Vladislav Mesiakaris, Konstantinos Al Tobi, Mohammed Boffo, Silvia Arsenijevic, Aleksandar Zuccarello, Pietro Giallongo, Cesarina Ferrante, Margherita Polosa, Riccardo Li Volti, Giovanni eng Research Support, Non-U.S. Gov't England 2021/12/19 Sci Rep. 2021 Dec 17; 11(1):24182. doi: 10.1038/s41598-021-03310-y" |
