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Mol Biol Cell

Title:		Reconstruction of the kinetochore during meiosis in fission yeast Schizosaccharomyces pombe
Author(s):		Hayashi A; Asakawa H; Haraguchi T; Hiraoka Y;
Address:		"Kansai Advanced Research Center, National Institute of Information and Communications Technology, 588-2 Iwaoka, Kobe 651-2492, Japan"
Journal Title:		Mol Biol Cell
Year:		2006
Volume:		20061011
Issue:		12
Page Number:		5173 - 5184
DOI:		10.1091/mbc.e06-05-0388
ISSN/ISBN:		1059-1524 (Print) 1059-1524 (Linking)
Abstract:		"During the transition from mitosis to meiosis, the kinetochore undergoes significant reorganization, switching from a bipolar to a monopolar orientation. To examine the centromere proteins that are involved in fundamental reorganization in meiosis, we observed the localization of 22 mitotic and 2 meiotic protein components of the kinetochore during meiosis in living cells of the fission yeast. We found that the 22 mitotic proteins can be classified into three groups: the Mis6-like group, the NMS (Ndc80-Mis12-Spc7) group, and the DASH group, based on their meiotic behavior. Mis6-like group proteins remain at the centromere throughout meiosis. NMS group proteins disappear from the centromere at the onset of meiosis and reappear at the centromere in two steps in late prophase. DASH group proteins appear shortly before metaphase of meiosis I. These observations suggest that Mis6-like group proteins constitute the structural basis of the centromere and that the NMS and DASH group proteins reassemble to establish the functional metaphase kinetochore. On the other hand, the meiosis-specific protein Moa1, which plays an important role in forming the meiotic monopolar kinetochore, is loaded onto the centromere significantly earlier than the NMS group, whereas another meiosis-specific protein, Sgo1, is loaded at times similar to the NMS group"
Keywords:		"Amino Acid Sequence Cell Cycle Proteins/metabolism Cell Survival Chromosomal Proteins, Non-Histone/metabolism Kinetochores/*metabolism Lipoproteins/metabolism *Meiosis Mitosis Models, Biological Molecular Sequence Data Multiprotein Complexes/metabolism Ph;"
Notes:		"MedlineHayashi, Aki Asakawa, Haruhiko Haraguchi, Tokuko Hiraoka, Yasushi eng Research Support, Non-U.S. Gov't 2006/10/13 Mol Biol Cell. 2006 Dec; 17(12):5173-84. doi: 10.1091/mbc.e06-05-0388. Epub 2006 Oct 11"