« Previous AbstractSurface chemistry of a pine-oil cleaner and other terpene mixtures with ozone on vinyl flooring tiles    Next Abstract"Pheromone-induced stimulation of hypothalamic gonadotropin-releasing hormone pulse generator in ovariectomized, estrogen-primed goats" »

Planta

Title:		Overexpression of geraniol synthase induces heat stress susceptibility in Nicotiana tabacum
Author(s):		Hamachi A; Nisihara M; Saito S; Rim H; Takahashi H; Islam M; Uemura T; Ohnishi T; Ozawa R; Maffei ME; Arimura GI;
Address:		"Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo, 125-8585, Japan. Iwate Biotechnology Research Center, Kitakami, Iwate, 024-0003, Japan. Department of Life Sciences and Systems Biology, Plant Physiology Unit, University of Turin, Via Quarello15/A, I-10135, Turin, Italy. College of Agriculture, Academic Institute, Shizuoka University, Shizuoka, 422-8529, Japan. Research Institute of Green Science and Technology, Shizuoka University, Shizuoka, 422-8529, Japan. Center for Ecological Research, Kyoto University, Otsu, 520-2113, Japan. Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo, 125-8585, Japan. garimura@rs.tus.ac.jp"
Journal Title:		Planta
Year:		2019
Volume:		20181126
Issue:		1
Page Number:		235 - 249
DOI:		10.1007/s00425-018-3054-z
ISSN/ISBN:		1432-2048 (Electronic) 0032-0935 (Linking)
Abstract:		"Transgenic tobacco plants overexpressing the monoterpene alcohol geraniol synthase exhibit hypersensitivity to thermal stress, possibly due to suppressed sugar metabolism and transcriptional regulation of genes involved in thermal stress tolerance. Monoterpene alcohols function in plant survival strategies, but they may cause self-toxicity to plants due to their hydrophobic and highly reactive properties. To explore the role of these compounds in plant stress responses, we assessed transgenic tobacco plants overexpressing the monoterpene alcohol geraniol synthase (GES plants). Growth, morphology and photosynthetic efficiency of GES plants were not significantly different from those of control plants (wild-type and GUS-transformed plants). While GES plants' direct defenses against herbivores or pathogens were similar to those of control plants, their indirect defense (i.e., attracting herbivore enemy Nesidiocoris tenuis) was stronger compared to that of control plants. However, GES plants were susceptible to cold stress and even more susceptible to extreme heat stress (50 degrees C), as shown by decreased levels of sugar metabolites, invertase activity and its products (Glc and Fru), and leaf starch granules. Moreover, GES plants showed decreased transcription levels of the WRKY33 transcription factor gene and an aquaporin gene (PIP2). The results of this study show that GES plants exhibit enhanced indirect defense ability against herbivores, but conversely, GES plants exhibit hypersensitivity to heat stress due to suppressed sugar metabolism and gene regulation for thermal stress tolerance"
Keywords:		"Arabidopsis Proteins/genetics/metabolism Gene Expression Regulation, Plant Phosphoric Monoester Hydrolases/genetics/*metabolism Plant Proteins/genetics/*metabolism Plants, Genetically Modified/genetics/*metabolism Tobacco/genetics/*metabolism Transcriptio;"
Notes:		"MedlineHamachi, Ashita Nisihara, Masahiro Saito, Shiori Rim, Hojun Takahashi, Hideyuki Islam, Monirul Uemura, Takuya Ohnishi, Toshiyuki Ozawa, Rika Maffei, Massimo E Arimura, Gen-Ichiro eng 16K07407/Japan Society for the Promotion of Science/ 18H04630/Grant-in-Aid for Scientific Research on Innovative Areas/ 18H04786/Grant-in-Aid for Scientific Research on Innovative Areas/ Germany 2018/11/28 Planta. 2019 Jan; 249(1):235-249. doi: 10.1007/s00425-018-3054-z. Epub 2018 Nov 26"