| Title: | Phospholipase D1 is required for efficient mating projection formation in Saccharomyces cerevisiae |
| Author(s): | Hairfield ML; Ayers AB; Dolan JW; |
| Address: | "Molecular, Cellular Biology and Pathobiology Program, Medical University of South Carolina, Charleston 29425, USA" |
| DOI: | 10.1111/j.1567-1364.2001.tb00038.x |
| ISSN/ISBN: | 1567-1356 (Print) 1567-1356 (Linking) |
| Abstract: | "Phospholipase D1 (PLD1) is an important enzyme involved in lipid signal transduction in eukaryotes. A role for PLD1 in signaling in Saccharomyces cerevisiae was examined. Pheromone response in yeast is controlled by a well-characterized protein kinase cascade. Loss of PLD1 activity was found to impair pheromone-induced changes in cellular morphology that result in formation of mating projections. The rate at which projections appeared following pheromone treatment was delayed, suggesting that PLD1 facilitates the execution of a rate-limiting step in morphogenesis. Mutants were found to be less sensitive to pheromone, again arguing that PLD1 is acting at a rate-limiting step. The fact that morphogenesis is most dramatically affected indicates that PLD1 functions primarily in the morphogenic branch of the pheromone response pathway" |
| Keywords: | "Culture Media Fungal Proteins/metabolism Gene Expression Regulation, Fungal Morphogenesis Pheromones/*metabolism Phospholipase D/genetics/*metabolism Saccharomyces cerevisiae/enzymology/*genetics/*physiology *Saccharomyces cerevisiae Proteins *Signal Tran;" |
| Notes: | "MedlineHairfield, M L Ayers, A B Dolan, J W eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 2003/04/19 FEMS Yeast Res. 2001 Dec; 1(3):225-32. doi: 10.1111/j.1567-1364.2001.tb00038.x" |
