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Methods Mol Biol


Title:Pheromone-induced expression of immediate early genes in the mouse vomeronasal sensory system
Author(s):Haga-Yamanaka S; Touhara K;
Address:"Stowers Institute for Medical Research, Kansas City, MO, USA"
Journal Title:Methods Mol Biol
Year:2013
Volume:1068
Issue:
Page Number:247 - 258
DOI: 10.1007/978-1-62703-619-1_18
ISSN/ISBN:1940-6029 (Electronic) 1064-3745 (Linking)
Abstract:"Immediate early genes (IEGs) are powerful tools for visualizing activated neurons and extended circuits that are stimulated by sensory input. Several kinds of IEGs (e.g., c-fos, egr-1) have been utilized for detecting activated receptor neurons in the pheromone sensory organ called the vomeronasal organ (VNO), as well as for mapping the neurons within the central nervous system (CNS) excited by pheromones.In this chapter, we describe the procedure for the detection of pheromone-induced neural activation in the VNO and CNS using the c-Fos immunostaining technique"
Keywords:"Amygdala/physiology Animals Female Genes, Immediate-Early/*genetics Mice Neurons/*physiology Olfactory Bulb/physiology Pheromones/metabolism Preoptic Area/physiology Proto-Oncogene Proteins c-fos/biosynthesis/*genetics/metabolism Septal Nuclei/physiology;"
Notes:"MedlineHaga-Yamanaka, Sachiko Touhara, Kazushige eng 2013/09/10 Methods Mol Biol. 2013; 1068:247-58. doi: 10.1007/978-1-62703-619-1_18"

 
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