Bedoukian   RussellIPM   RussellIPM   Piezoelectric Micro-Sprayer


Home
Animal Taxa
Plant Taxa
Semiochemicals
Floral Compounds
Semiochemical Detail
Semiochemicals & Taxa
Synthesis
Control
Invasive spp.
References

Abstract

Guide

Alphascents
Pherobio
InsectScience
E-Econex
Counterpart-Semiochemicals
Print
Email to a Friend
Kindly Donate for The Pherobase

« Previous AbstractVolatile Organic Compound (VOC) Profiles of Different Trichoderma Species and Their Potential Application    Next AbstractParticle-based simulations reveal two positive feedback loops allow relocation and stabilization of the polarity site during yeast mating »

J Chromatogr B Analyt Technol Biomed Life Sci


Title:Rapid detection of sepsis in rats through volatile organic compounds in breath
Author(s):Guaman AV; Carreras A; Calvo D; Agudo I; Navajas D; Pardo A; Marco S; Farre R;
Address:"Institute for Bioengineering of Catalonia (IBEC), Baldiri i Rexach 4 08028-BCN, Spain"
Journal Title:J Chromatogr B Analyt Technol Biomed Life Sci
Year:2012
Volume:20111210
Issue:
Page Number:76 - 82
DOI: 10.1016/j.jchromb.2011.12.001
ISSN/ISBN:1873-376X (Electronic) 1570-0232 (Linking)
Abstract:"BACKGROUND: Sepsis is one of the main causes of death in adult intensive care units. The major drawbacks of the different methods used for its diagnosis and monitoring are their inability to provide fast responses and unsuitability for bedside use. In this study, performed using a rat sepsis model, we evaluate breath analysis with Ion Mobility Spectrometry (IMS) as a fast, portable and non-invasive strategy. METHODS: This study was carried out on 20 Sprague-Dawley rats. Ten rats were injected with lipopolysaccharide from Escherichia coli and ten rats were IP injected with regular saline. After a 24-h period, the rats were anaesthetized and their exhaled breaths were collected and measured with IMS and SPME-gas chromatography/mass spectrometry (SPME-GC/MS) and the data were analyzed with multivariate data processing techniques. RESULTS: The SPME-GC/MS dataset processing showed 92% accuracy in the discrimination between the two groups, with a confidence interval of between 90.9% and 92.9%. Percentages for sensitivity and specificity were 98% (97.5-98.5%) and 85% (84.6-87.6%), respectively. The IMS database processing generated an accuracy of 99.8% (99.7-99.9%), a specificity of 99.6% (99.5-99.7%) and a sensitivity of 99.9% (99.8-100%). CONCLUSIONS: IMS involving fast analysis times, minimum sample handling and portable instrumentation can be an alternative for continuous bedside monitoring. IMS spectra require data processing with proper statistical models for the technique to be used as an alternative to other methods. These animal model results suggest that exhaled breath can be used as a point-of-care tool for the diagnosis and monitoring of sepsis"
Keywords:"Animals Breath Tests/instrumentation/*methods Databases, Factual Disease Models, Animal Escherichia coli Gas Chromatography-Mass Spectrometry/methods Lipopolysaccharides Male Mass Spectrometry/*methods Multivariate Analysis Rats Rats, Sprague-Dawley Repro;"
Notes:"MedlineGuaman, Ana V Carreras, Alba Calvo, Daniel Agudo, Idoya Navajas, Daniel Pardo, Antonio Marco, Santiago Farre, Ramon eng Research Support, Non-U.S. Gov't Netherlands 2012/01/03 J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Jan 15; 881-882:76-82. doi: 10.1016/j.jchromb.2011.12.001. Epub 2011 Dec 10"

 
Back to top
 
Citation: El-Sayed AM 2024. The Pherobase: Database of Pheromones and Semiochemicals. <http://www.pherobase.com>.
© 2003-2024 The Pherobase - Extensive Database of Pheromones and Semiochemicals. Ashraf M. El-Sayed.
Page created on 17-11-2024