| Title: | Secretion is required for late events in the cell-fusion pathway of mating yeast |
| Address: | "Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, 615 N. Wolfe Street, Baltimore, MD 21205. egrote@jhsph.edu" |
| ISSN/ISBN: | 1477-9137 (Electronic) 0021-9533 (Linking) |
| Abstract: | "Secretory vesicles accumulate adjacent to the contact site between the two cells of a yeast mating pair before they fuse, but there is no direct evidence that secretion is required to complete fusion. In this study, temperature-sensitive secretion (sec(ts)) mutants were used to investigate the role of secretion in yeast cell fusion. Cell fusion arrested less than 5 minutes after inhibiting secretion. This rapid fusion arrest was not an indirect consequence of reduced mating pheromone signaling, mating-pair assembly or actin polarity. Furthermore, secretion was required to complete cell fusion when it was transiently inhibited by addition and removal of the lipophilic styryl dye, FM4-64. These results indicate that ongoing secretion is required for late events in the cell-fusion pathway, which include plasma-membrane fusion and the completion of cell-wall remodeling, and they demonstrate a just-in-time delivery mechanism for the cell-fusion machinery" |
| Keywords: | "Cells, Cultured Genetic Complementation Test Membrane Fusion/drug effects/genetics Membrane Proteins/genetics Mutation/genetics Pyridinium Compounds/pharmacology Quaternary Ammonium Compounds/pharmacology Reproduction Saccharomyces cerevisiae Proteins/gen;" |
| Notes: | "MedlineGrote, Eric eng Research Support, Non-U.S. Gov't England 2010/05/13 J Cell Sci. 2010 Jun 1; 123(Pt 11):1902-12. doi: 10.1242/jcs.066662. Epub 2010 May 11" |
