| Title: | Yeast alpha-factor and somatostatin enhance binding of [3H]estradiol to proteins in rat pancreas and Saccharomyces cerevisiae |
| Author(s): | Grossman A; Klein H; Becker JM; Naider F; |
| DOI: | 10.1016/0022-4731(86)90239-6 |
| ISSN/ISBN: | 0022-4731 (Print) 0022-4731 (Linking) |
| Abstract: | "Pancreatic tissue contains an [3H]estradiol-binding protein that requires a coligand in the steroid-binding reaction. The endogenous coligand appears to be the tetradecapeptide somatostatin. Yeast alpha-factor, a tridecapeptide pheromone that induces conjugation between haploid cells of opposite mating type, was found to be as effective as somatostatin in enhancing specific binding of [3H]estradiol to partially purified pancreatic protein. Supernatant fractions from yeast cells also contain an [3H]estradiol-binding protein. alpha-Factor can enhance specific binding of [3H]estradiol to such yeast fractions. Somatostatin, somatostatin analogues, and an analogue of alpha-factor enhanced binding of [3H]estradiol but did not inhibit cell growth or induce morphological changes in S. cerevisiae. Thus, it appears that coligand-requiring [3H]estradiol-binding activity and mating in yeast are not directly related" |
| Keywords: | "Amino Acid Sequence Animals Estradiol/*metabolism Kinetics Mating Factor Pancreas/drug effects/*metabolism Peptides/*pharmacology Proteins/*metabolism Rats Receptors, Estradiol/metabolism Saccharomyces cerevisiae/drug effects/*metabolism Somatostatin/*pha;" |
| Notes: | "MedlineGrossman, A Klein, H Becker, J M Naider, F eng AM 20084/AM/NIADDK NIH HHS/ GM 22086/GM/NIGMS NIH HHS/ GM 30439/GM/NIGMS NIH HHS/ etc. Comparative Study Research Support, U.S. Gov't, P.H.S. England 1986/09/01 J Steroid Biochem. 1986 Sep; 25(3):299-304. doi: 10.1016/0022-4731(86)90239-6" |
