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Molecules

Title:		Yeast Smell Like What They Eat: Analysis of Volatile Organic Compounds of Malassezia furfur in Growth Media Supplemented with Different Lipids
Author(s):		Gonzalez M; Celis AM; Guevara-Suarez MI; Molina J; Carazzone C;
Address:		"Laboratory of Advanced Analytical Techniques in Natural Products (LATNAP), Universidad de los Andes, Cra 1 No. 18A-12, Bogota 111711, Cundinamarca, Colombia. mabel.c.gonzalez@uniandes.edu.co. Grupo de Investigacion Celular y Molecular de Microorganismos Patogenos (CeMoP), Universidad de los Andes, Cra 1 No. 18A-12, Bogota 111711, Cundinamarca, Colombia. acelis@uniandes.edu.co. Laboratorio de Micologia y Fitopatologia (LAMFU), Universidad de los Andes, Cra 1 No. 18A-12, Bogota 111711, Cundinamarca, Colombia. acelis@uniandes.edu.co. Grupo de Investigacion Celular y Molecular de Microorganismos Patogenos (CeMoP), Universidad de los Andes, Cra 1 No. 18A-12, Bogota 111711, Cundinamarca, Colombia. mi.guevara34@uniandes.edu.co. Laboratorio de Micologia y Fitopatologia (LAMFU), Universidad de los Andes, Cra 1 No. 18A-12, Bogota 111711, Cundinamarca, Colombia. mi.guevara34@uniandes.edu.co. Centro de Investigaciones en Microbiologia y Parasitologia Tropical (CIMPAT), Universidad de los Andes, Cra 1 No. 18A-12, Bogota 111711, Cundinamarca, Colombia. jmolina@uniandes.edu.co. Laboratory of Advanced Analytical Techniques in Natural Products (LATNAP), Universidad de los Andes, Cra 1 No. 18A-12, Bogota 111711, Cundinamarca, Colombia. c.carazzone@uniandes.edu.co"
Journal Title:		Molecules
Year:		2019
Volume:		20190124
Issue:		3
Page Number:		-
DOI:		10.3390/molecules24030419
ISSN/ISBN:		1420-3049 (Electronic) 1420-3049 (Linking)
Abstract:		"Malassezia furfur is part of the human skin microbiota. Its volatile organic compounds (VOCs) possibly contribute to the characteristic odour in humans, as well as to microbiota interaction. The aim of this study was to investigate how the lipid composition of the liquid medium influences the production of VOCs. Growth was performed in four media: (1) mDixon, (2) oleic acid (OA), (3) oleic acid + palmitic acid (OA+PA), and (4) palmitic acid (PA). The profiles of the VOCs were characterized by HS-SPME/GC-MS in the exponential and stationary phases. A total number of 61 VOCs was found in M. furfur, among which alkanes, alcohols, ketones, and furanic compounds were the most abundant. Some compounds previously reported for Malassezia (gamma-dodecalactone, 3-methylbutan-1-ol, and hexan-1-ol) were also found. Through our experiments, using univariate and multivariate unsupervised (Hierarchical Cluster Analysis (HCA) and Principal Component Analysis (PCA)) and supervised (Projection to Latent Structures Discriminant Analysis (PLS-DA)) statistical techniques, we have proven that each tested growth medium stimulates the production of a different volatiles profile in M. furfur. Carbon dioxide, hexan-1-ol, pentyl acetate, isomer5 of methyldecane, dimethyl sulphide, undec-5-ene, isomer2 of methylundecane, isomer1 of methyldecane, and 2-methyltetrahydrofuran were established as differentiating compounds among treatments by all the techniques. The significance of our findings deserves future research to investigate if certain volatile profiles could be related to the beneficial or pathogenic role of this yeast"
Keywords:		Culture Media/chemistry Gas Chromatography-Mass Spectrometry Lipid Metabolism Lipids/chemistry Malassezia/*chemistry/metabolism Metabolome Metabolomics/methods Solid Phase Microextraction Volatile Organic Compounds/*analysis/chemistry/*isolation & purific;
Notes:		"MedlineGonzalez, Mabel Celis, Adriana M Guevara-Suarez, Marcela I Molina, Jorge Carazzone, Chiara eng FAPA Project of Dr. Chiara Carazzone/Facultad de Ciencias, Universidad de los Andes/ 120465741393/Departamento Administrativo de Ciencia, Tecnologia e Innovacion/ 120456934423/Departamento Administrativo de Ciencia, Tecnologia e Innovacion/ announcement N. 757 Doctorados Nacionales/Departamento Administrativo de Ciencia, Tecnologia e Innovacion/ NFP-phd.14/99/Netherlands Fellowship Program/ Switzerland 2019/01/27 Molecules. 2019 Jan 24; 24(3):419. doi: 10.3390/molecules24030419"