| Title: | Organic metabolites in exhaled human breath--a multivariate approach for identification of biomarkers in lung disorders |
| Author(s): | Gaspar EM; Lucena AF; Duro da Costa J; Chaves das Neves H; |
| Address: | "Chemistry Department, CQFB-Requimte, Faculty of Sciences and Technology, New University of Lisbon, Quinta da Torre, 2825-114 Monte da Caparica, Portugal" |
| DOI: | 10.1016/j.chroma.2008.10.125 |
| ISSN/ISBN: | 1873-3778 (Electronic) 0021-9673 (Linking) |
| Abstract: | "The gas chromatographic profiles of exhaled air from lung cancer patients have been investigated. The breath from healthy volunteers, smokers and non-smokers, and lung cancer patients without treatment and under radio and/or chemotherapy, was collected using Tedlar bags. Different profiles for healthy people and cancer patients could be recognized by multivariate analysis and significant diagnostic compounds could be established. Target compounds showed to be linear and branched hydrocarbons between C(14) and C(24). Solid phase microextraction (SPME) coupled to gas chromatography mass spectrometry GC-(TOF)-MS was used. The method showed good precision (RSD below 26%) and limit of detection ranged from 0.04 to 8.0ppb. These findings show a high potential for establishment of laboratorial screening methods. Validation studies in a larger number of patients are being done" |
| Keywords: | Biomarkers/analysis/metabolism *Exhalation Gas Chromatography-Mass Spectrometry/*methods Humans Hydrocarbons/*analysis/isolation & purification Lung Neoplasms/*diagnosis/metabolism Sensitivity and Specificity Smoking/metabolism Solid Phase Extraction Vola; |
| Notes: | "MedlineGaspar, Elvira M Lucena, Ana F Duro da Costa, J Chaves das Neves, H eng Validation Study Netherlands 2008/11/28 J Chromatogr A. 2009 Apr 3; 1216(14):2749-56. doi: 10.1016/j.chroma.2008.10.125. Epub 2008 Nov 12" |
