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BMC Genet

Title:		RNA-seq analysis of lncRNA-controlled developmental gene expression during puberty in goat & rat
Author(s):		Gao X; Ye J; Yang C; Luo L; Liu Y; Ding J; Zhang Y; Ling Y; Huang W; Zhang X; Zhang K; Li X; Zhou J; Fang F; Cao Z;
Address:		"Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Science and Technology, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, Anhui, China. Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, 130 Changjiang West Road, Hefei, 230036, Anhui, China. Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, Anhui, China. Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Science and Technology, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, Anhui, China. fgfang@163.com. Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, 130 Changjiang West Road, Hefei, 230036, Anhui, China. fgfang@163.com. Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, Anhui, China. fgfang@163.com. Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Science and Technology, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, Anhui, China. zubingcao@ahau.edu.cn. Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, 130 Changjiang West Road, Hefei, 230036, Anhui, China. zubingcao@ahau.edu.cn. Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, Anhui, China. zubingcao@ahau.edu.cn"
Journal Title:		BMC Genet
Year:		2018
Volume:		20180402
Issue:		1
Page Number:		19 -
DOI:		10.1186/s12863-018-0608-9
ISSN/ISBN:		1471-2156 (Electronic) 1471-2156 (Linking)
Abstract:		"BACKGROUND: Puberty is a pivotal stage in female animal development, and marks the onset of reproductive capability. However, little is known about the function of lncRNAs (long noncoding RNAs) in puberty. Therefore, RNA-seq analysis were performed between goats and rats to clarify the roles of lncRNAs and mRNAs in the onset of puberty. RESULTS: In the present study, the length of lncRNAs, the length of the open reading frame and the exon count were compared between the two species. Furthermore, functional annotation analysis based on Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) analysis of lncRNAs target genes and differentially expressed mRNA demonstrated the significantly enriched terms, such as AMPK signaling pathway, oxytocin signaling pathway, insulin secretion as well as pheromone receptor activity, and some other signaling pathways which were involved in the regulation of female puberty. Moreover, our results of siRNA interference in vitro showed the candidate lncRNA XLOC_446331 may play a crucial role in regulating female puberty. CONCLUSION: In conclusion, the RNA-seq analysis between goat and rat provide novel candidate regulators for genetic and molecular studies on female puberty"
Keywords:		"Animals Female Gene Expression Profiling *Gene Expression Regulation, Developmental Gene Ontology Genes, Developmental Goats/*genetics/*growth & development RNA, Long Noncoding/*genetics RNA, Messenger/genetics Rats Sequence Analysis, RNA Sexual Maturatio;"
Notes:		"MedlineGao, Xiaoxiao Ye, Jing Yang, Chen Luo, Lei Liu, Ya Ding, Jianping Zhang, Yunhai Ling, Yinghui Huang, Weiping Zhang, Xiaorong Zhang, Kaifa Li, Xiumei Zhou, Jie Fang, Fugui Cao, Zubing eng Research Support, Non-U.S. Gov't England 2018/04/04 BMC Genet. 2018 Apr 2; 19(1):19. doi: 10.1186/s12863-018-0608-9"