| Title: | Selection of Reference Genes for qRT-PCR Analysis in Lentinula edodes after Hot-Air Drying |
| Author(s): | Gao S; Wang G; Huang Z; Lei X; Bian Y; Liu Y; Huang W; |
| Address: | "College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China. 13720161459@163.com. Institute of Applied Mycology, Plant Science and Technology College, Huazhong Agricultural University, Wuhan, Hubei 430070, China. wgzhau@163.com. College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China. HuangZhiCheng1210@163.com. College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China. xiaoyulei1988@126.com. Institute of Applied Mycology, Plant Science and Technology College, Huazhong Agricultural University, Wuhan, Hubei 430070, China. bianyinbing@mail.hzau.edu.cn. College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China. yingliu@mail.hzau.edu.cn. College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China. huangwen@mail.hzau.edu.cn" |
| DOI: | 10.3390/molecules24010136 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "Volatile sulfur compounds gradually develop in Lentinula edodes after hot-air drying, and many genes are involved in the generation of these sulfur compounds. The expression stability of reference genes may vary in a particular experimental treatment when analyzing their expressions by quantitative real-time polymerase chain reaction (qRT-PCR). In this study, the expression profile of 17 candidate genes was assessed in L. edodes under treatment at 50 degrees C for 0, 1, 2, and 3 h, and the expression stability of each reference gene was analyzed by three statistical algorithms, including geNorm, NormFinder, and BestKeeper. Results indicated that the two optimal reference genes for mycelium and fruiting body were CAC and DAHP as well as CAC and NUP, respectively. Additionally, CAC and DAHP were found to be the two most stable reference genes across the mycelium and fruiting body set. Our results will provide a genetic foundation for further research on the metabolism genes of sulfur compounds in L. edodes" |
| Keywords: | "Algorithms Desiccation/*methods Fungal Proteins/*genetics Gene Expression Profiling/standards Gene Expression Regulation, Developmental Gene Expression Regulation, Fungal Hot Temperature Real-Time Polymerase Chain Reaction/*standards Reference Standards S;" |
| Notes: | "MedlineGao, Shuangshuang Wang, Gangzheng Huang, Zhicheng Lei, Xiaoyu Bian, Yinbing Liu, Ying Huang, Wen eng 31601434/Natural Science Foundation of China/ 2017ABA148/Major Projects of Technological Innovation of Hubei Province/ 2016T90701/China Postdoctoral Science Foundation/ Switzerland 2019/01/04 Molecules. 2018 Dec 31; 24(1):136. doi: 10.3390/molecules24010136" |
