| Title: | Revealing an unrecognized role of free ammonia in sulfur transformation during sludge anaerobic treatment |
| Author(s): | Fu Q; Long S; Xu Y; Wang Y; Yang B; He D; Li X; Liu X; Lu Q; Wang D; |
| Address: | "College of Environmental Science and Engineering and Key Laboratory of Environmental Biology and Pollution Control (Ministry of Education), Hunan University, Changsha 410082, PR China. Zhongye Changtian International Engineering Co., Ltd., Changsha 410205, PR China. College of Environmental Science and Engineering and Key Laboratory of Environmental Biology and Pollution Control (Ministry of Education), Hunan University, Changsha 410082, PR China. Electronic address: dongbowang@hnu.edu.cn" |
| DOI: | 10.1016/j.jhazmat.2023.131305 |
| ISSN/ISBN: | 1873-3336 (Electronic) 0304-3894 (Linking) |
| Abstract: | "Free ammonia (FA), the unionized form of ammonium, is presented in anaerobic fermentation of waste activated sludge (WAS) at high levels. However, its potential role in sulfur transformation, especially H(2)S production, during WAS anaerobic fermentation process was unrecognized previously. This work aims to unveil how FA affects anaerobic sulfur transformation in WAS anaerobic fermentation. It was found that FA significantly inhibited H(2)S production. With an increase of FA from 0.04 to 159 mg/L, H(2)S production reduced by 69.9%. FA firstly attacked tyrosine-like proteins and aromatic-like proteins in sludge EPSs, with CO groups being responded first, which decreased the percentage of alpha-helix/(beta-sheet + random coil) and destroyed hydrogen bonding networks. Cell membrane potential and physiological status analysis showed that FA destroyed membrane integrity and increased the ratio of apoptotic and necrotic cells. These destroyed sludge EPSs structure and caused cell lysis, thus strongly inhibited the activities of hydrolytic microorganisms and sulfate reducing bacteria. Microbial analysis showed that FA reduced the abundance of functional microbes (e.g., Desulfobulbus and Desulfovibrio) and genes (e.g., MPST, CysP, and CysN) involved in organic sulfur hydrolysis and inorganic sulfate reduction. These findings unveil an actually existed but previously overlooked contributor to H(2)S inhibition in WAS anaerobic fermentation" |
| Keywords: | "*Ammonia/metabolism Sewage/chemistry Anaerobiosis Fermentation *Ammonium Compounds Fatty Acids, Volatile/chemistry Anaerobic fermentation Free ammonia Hydrogen sulfide (H(2)S) Waste activated sludge (WAS);" |
| Notes: | "MedlineFu, Qizi Long, Sha Xu, Yunhao Wang, Yan Yang, Bentao He, Dandan Li, Xuemei Liu, Xuran Lu, Qi Wang, Dongbo eng Research Support, Non-U.S. Gov't Netherlands 2023/04/02 J Hazard Mater. 2023 Jun 15; 452:131305. doi: 10.1016/j.jhazmat.2023.131305. Epub 2023 Mar 28" |
